丙咪嗪对脑外伤后大鼠海马内神经元再生的促进作用

Imipramine promotes regeneration of hippocampus neuron after traumatic brain injury in rat

目的观察丙咪嗪对脑外伤后大鼠海马内神经元再生的影响,以探讨其治疗机制。方法成年雄性SD大鼠40只,随机均分为损伤治疗组、损伤对照组、未损伤治疗组和未损伤对照组。采用鼠侧向液压冲击脑损伤装置建立急性脑创伤模型,丙咪嗪治疗2个月后处死动物,观察各组大鼠海马内新生细胞和神经元的数量。结果大鼠海马内新生细胞和神经元的数量损伤组均明显高于未损伤组(P<0.01),而且无论有无脑创伤,丙咪嗪治疗组均高于相应的对照组(P<0.05)。结论丙咪嗪能够与颅脑创伤协同作用共同促进海马内神经元的再生。

Objective To explore the potential mechanism of efficacy of imipramine treatment in the regeneration of hippoeampus neuron after traumatic brain injury in rat. Methods Lateral hydraulic battering injury （HBI） was used to induce acute brain injury in rats. Forty male SD rats were randomly assigned to four groups： HBI with imipramine treatment group, HBI without treatment group, sham injury with imipramine treatment group and sham injury without treatment group. Animals of all the four groups were sacrificed for histo logical evaluation two months after treatment. Proliferating cells and neurons in hippocampus were counted and compared between groups. Results Proliferating cells and neurons were found to be obviously increased in all injured rats compared with sham injured animals （P〈0.01）. Moreover, either in injury or sham group, a substantial neurogenesis was observed in imipramine-treated rats compared with the corresponding controls. Conclusion Treatment with imipramine for traumatic brain injury may promote neurogenesis of hippocampus neuron.