维拉帕米对外照射导致的小细胞肺癌细胞系耐药性的逆转作用

Reversal effect of verapamil on the drug resistance of NCI-H446 cell line induced by radiation

目的观察维拉帕米对分次外照射引发的小细胞肺癌细胞系NCI-H446耐药性的逆转作用。方法采用GWGP80型远距离60Co治疗机对进入指数生长期的NCI-H446细胞进行分次外照射,总照射剂量为50Gy。用不同浓度的丝裂霉素(MMC)干扰,观察外照射前后NCI-H446细胞存活率的变化;同时观察在上述干扰条件不变的前提下,加入逆转剂维拉帕米后其存活率的变化。结果在相同浓度MMC干扰下,照射组细胞的存活率均明显高于未照射组(P<0.01);加入逆转剂维拉帕米后,两组细胞的存活率差别不大(P>0.05)或者照射组细胞的存活率反而明显低于未照射组(P<0.01)。结论经逆转剂维拉帕米作用后,照射组细胞的耐药性几乎完全被逆转甚至对MMC更敏感。

Objective To investigate the reversal effect of verapamil on the drug resistance of NCI-H 446 small cell lung cancer cell line induced by fractionated radiation. Methods Exponentially grown NCI-H 446 cells were exposed to total radiation dose of 50 Gy, which was given in 25 fractions of 2 Gy per fraction. The survival rate of NCI-H 446 cell line, before and after fractional radiation, was observed after adding different concentrations of mitomycin C （MMC）. The change in survival rate of NCI-H 446 cell line was also observed while verapamil was added additionally to the above. Results The survival rate of the radiated cell line was higher than that of the unradiated cell line under the same concentration of MMC （P〈0. 01）. After adding the reversal agent of verapamil to the radiated cell line and the unradiated cell line, the survival rates of these two groups were almost equal （P〉0. 05）, or the survival rate of radiated cell line was obviously lower than that of unradiated cell （P〈0.01）. Conclusion The drug resistance of radiated cell line is almost reversed, or the radiated cell line becomes more sensitive to MMC after adding the reversal drug verapamil.