小麦—簇毛麦染色体代换系、易位系特异蛋白组分的双向电泳比较分析(英文)

Comparison of the Protein Composition and Analysis of the Specific Protein from Wheat-Haynaldid villosa Chromosome Substitution Line and Translocation Line by 2D-PAGE

利用双向电泳技术,对栽培小麦(AABBDD)、染色体代换系(6V/6A)、易位系(6VS/6AL)、(6VS/6DL)和簇毛麦(VV)的叶片全蛋白进行了比较研究。在栽培小麦、代换系和两个易位系中检测到超过350个蛋白组分,它们的分子量范围是10-110 KD,等电点在4.5-8.6之间。栽培小麦、6V/6A、6VS/6AL、与6VS/6DL之间的双向电泳谱型极为相似,但与簇毛麦不同。在代换系、两个易位系和簇毛麦中检测到了特异蛋白组分16 KD/pI5.0,而在栽培小麦中未检测到该组分,这些结果表明16 KD/pI5.0蛋白可能定位于簇毛麦V染色体短臂上。

With 2D-PAGE techniques, comparison of leaf protein in cultivar wheat （AABBDD）, Wheat-Haynaldia villosa chromosome substitution line （ 6V/6A ）, translocation lines （ 6VS/6AL ）, （6VS/6DL） and H. villosa （VV） have been made in this study. Over 350 protein species were detected in the leaf of cuhivar wheat, chromosome substitution line and two translocation lines. Their molecular weight ranged from 10 - 110 KD and pI from 4. 5 - 8.6. 2D-PAGE patterns of leaf protein are almost the same among cultivar wheat, 6V/6A, 6VS/6AL, 6VS/6DL; but different from H. villosa. A specific protein （16 KD/pI5.0） was found in substitution line, two translocation lines and H. villosa, but not found in the cuhivar wheat. These results suggested that the protein （ 16 KD/pI5.0） may be located in the short arm of V chromosome of H. villosa.