HPLC-ELSD法在刺五加药材糖类成分分析中的应用

Analysis of Sugars in Acanthopanax senticosus(Rupr.et Maxim.) by HPLC with ELSD Detection

建立一种高效、快速的高效液相色谱—蒸发光散射检测法(HPLC-ELSD)分析刺五加药材中糖类成分—葡萄糖及蔗糖组分。采用CHROMATOREX NH2柱为固定相;以乙腈:水为84.5:15.5(V/V)为流动相,流速1.0 mL.min-1;漂移管温度为100℃;氮气流速为2.5 L.min-1。在上述色谱条件下,葡萄糖和蔗糖的线性范围分别为0.023 2-0.116 0 mg.mL-1及0.099 6-0.496 5 mg.mL-1;且该二种糖的检测限分别(信/噪=3)为3.87 ng和3.31 ng。本方法无须进行糖衍生化而直接用于刺五加药材中糖类成分的分离分析。

A simple and rapid HPLC coupled with Evaporative Light Scattering Detection （ELSD） method was developed for the determination of glucose and sucrose in Acanthopanax senticosus （ Rupr. et Maxim. ）. The HPLC separation was performed on a CHROMATOREX NH2 column using acetonitrilewater （84. 5 ： 15.5, v/v） as mobile phase at a flow rate of 1 mL·min^-1The drift tube temperature of ELSD was set at 100℃, and with the nitrogen flow-rate of 2. 5 mL·min^-1 The linear ranges were from 0. 023 2 to 0. 116 0 mg · mL^-1 for glucose, and from 0. 099 6 to 0. 496 5 mg · mL^-1 for sucrose. All regression coefficients were more than 0. 999 0. The detection limits （ Signal/Noise = 3 ） obtained were 3.87 ng and 3.31 ng for glucose and sucrose, respectively. The method was first used for separation and determination of sugars in Traditional Chinese herb Acanthopanax senticosus （ Rupr. et Maxim. ） without prior derivatization, and the results were satisfactory.