应用聚合酶链反应技术克隆猪源大肠杆菌耐热肠毒素基因

Cloning of an Esherichia coli heat stable enterotoxin gene from porcine origin by PCR

以质粒ＤＮＡ为模板，利用聚合酶链反应（ＰＣＲ）技术扩增和克隆了猪大肠杆菌耐热毒素基因ＤＮＡ片段；核苷酸序列分析表明。

The heat stable enterotoxin gene of E.coli from porcine origin was synthesized by polymerase chain reaction and then cloned in pGEM 3zf(+),The nucleotide sequence data obtained were corresponded to the sequence data obtained were corresponded to the sequence of the STla gene,in which it lacked the last three codons of the CooH terminus.