摘要
目的:检测不同类型牙周炎患者非刺激性全唾液中白细胞介素1β(interleukin-1β,IL-1β)和基质金属蛋白酶-8 (matrix metalloproteinase-8,MMP-8)水平,探讨其作为牙周炎特异性标志物的可能性。方法:从门诊选取28例广泛型重度牙周炎和广泛型侵袭性牙周炎患者以及24例正常对照,采集非刺激性全唾液,然后用IL-1β和MMP-8的ELISA试剂盒分别检测其唾液含量。对所得数据进行单因素方差分析。结果:唾液IL-1β含量在广泛型重度牙周炎组、广泛型侵袭性牙周炎组和对照组中分别为(144.40±150.70)pg/ml,(72.56±69.36)pg/ml,(65.96±71.18)pg/ml,3组之间无显著差异(P>0.05);MMP-8水平在广泛型重度牙周炎和广泛型侵袭性牙周炎2组中均显著增高,分别为(576.89±559.24)ng/ml和(420.93±533.73)ng/ml,与对照组(151.49±216.38)ng/ml比较有显著差异(P<0.05),而2组之间无显著差异(P>0.05);同时,IL-1β、MMP-8与PD、AL、BOP和唾液流量之间无显著相关性(P>0.05)。结论:唾液中的MMP-8水平可能成为牙周炎诊断的分子标志物,IL-1B则需要进一步的研究来证实。
PURPOSE: To detect the role of IL-1β and MMP-8 by ELISA in whole unstimulated saliva of patients with different type of periodontitis and to determine whether they are possible specific biomarkers of periodontitis. METHODS: A total of 80 saliva samples were collected from 28 GCP patients, 28 GAgP patients and 24 healthy subjects. The levels of IL-1β and MMP-8 were respectively measured by ELISA kits. The data were analysed statistically with one-way ANOVA. RESULTS: The mean salivary levels of IL-1β in GCP group,GAgP group and control group were 144.40 pg/ml±150.70 pg/ml,72.56 pg/ml±69.36 pg/ml and 65.96 pg/ml±71.18 pg/ml respectively, There was no significance difference between the three groups (P〉0.05); GCP and GAgP groups presented significantly elevated salivary levels of MMP-8 than that of the control group, which was (576.89±559.24) ng/ml, (420.93±533.73)ng/ml and (151.49±216.38) ng/ml respectively (P〈 0.05), while there was no significant difference between GCP and GAgP group(P〉0.05). CONCLUSION: Salivary levels of MMP-8 may be a biomarker for diagnosis for periodontitis while IL-1β needs to be further testified. Supported by National "Tenth Five-Year"Key Research Project of Science and Technology (Grant No.2004BA720A26).
出处
《上海口腔医学》
CAS
CSCD
2007年第2期127-130,共4页
Shanghai Journal of Stomatology
基金
科技部"十五"科技攻关项目(2004BA720A26)