牙周炎患者非刺激性全唾液中IL-1β和MMP-8的检测及意义

Detection and significance of IL-1βand MMP-8 in patients with periodontitis of whole unstimulated saliva

目的:检测不同类型牙周炎患者非刺激性全唾液中白细胞介素1β(interleukin-1β,IL-1β)和基质金属蛋白酶-8 (matrix metalloproteinase-8,MMP-8)水平,探讨其作为牙周炎特异性标志物的可能性。方法:从门诊选取28例广泛型重度牙周炎和广泛型侵袭性牙周炎患者以及24例正常对照,采集非刺激性全唾液,然后用IL-1β和MMP-8的ELISA试剂盒分别检测其唾液含量。对所得数据进行单因素方差分析。结果:唾液IL-1β含量在广泛型重度牙周炎组、广泛型侵袭性牙周炎组和对照组中分别为(144．40±150．70)pg／ml,(72．56±69．36)pg／ml,(65．96±71．18)pg／ml,3组之间无显著差异(P>0．05);MMP-8水平在广泛型重度牙周炎和广泛型侵袭性牙周炎2组中均显著增高,分别为(576．89±559．24)ng／ml和(420．93±533．73)ng/ml,与对照组(151．49±216．38)ng／ml比较有显著差异(P<0．05),而2组之间无显著差异(P>0．05);同时,IL-1β、MMP-8与PD、AL、BOP和唾液流量之间无显著相关性(P>0．05)。结论:唾液中的MMP-8水平可能成为牙周炎诊断的分子标志物,IL-1B则需要进一步的研究来证实。

PURPOSE： To detect the role of IL-1β and MMP-8 by ELISA in whole unstimulated saliva of patients with different type of periodontitis and to determine whether they are possible specific biomarkers of periodontitis. METHODS： A total of 80 saliva samples were collected from 28 GCP patients, 28 GAgP patients and 24 healthy subjects. The levels of IL-1β and MMP-8 were respectively measured by ELISA kits. The data were analysed statistically with one-way ANOVA. RESULTS： The mean salivary levels of IL-1β in GCP group,GAgP group and control group were 144.40 pg/ml±150.70 pg/ml,72.56 pg/ml±69.36 pg/ml and 65.96 pg/ml±71.18 pg/ml respectively, There was no significance difference between the three groups （P〉0.05）; GCP and GAgP groups presented significantly elevated salivary levels of MMP-8 than that of the control group, which was （576.89±559.24） ng/ml, （420.93±533.73）ng/ml and （151.49±216.38） ng/ml respectively （P〈 0.05）, while there was no significant difference between GCP and GAgP group（P〉0.05）. CONCLUSION： Salivary levels of MMP-8 may be a biomarker for diagnosis for periodontitis while IL-1β needs to be further testified. Supported by National ＂Tenth Five-Year＂Key Research Project of Science and Technology （Grant No.2004BA720A26）.