Rh-bFGF与Bio-Oss骨胶原联合促进牙周骨缺损再生的实验研究

Combination use of rh-bFGF and Bio-Oss collagen to enhance periodontal regeneration: An experimental study in dogs

目的:评价重组人成纤维细胞生长因子(rh-bFGF)与牛无机骨胶原(Bio-Oss collagen)复合物和Bio-Gide联合应用对犬牙周组织再生的作用。方法:选用4只成年杂种犬,在双侧上、下颌前磨牙缺失区的近、远中牙槽嵴手术制备5mm×5mm×5mm的3壁骨内缺损。采用3种不同方法进行处理,实验组1:rh-bFGF与Bio-Oss胶原复合物植入, Bio-Gide覆盖;实验组2:Bio-Oss胶原复合物植入,Bio-Gide覆盖;对照组:单纯Bio-Gide覆盖。根据临床、影像学和组织学测量指标对治疗结果进行评价。采用SAS 6．2统计软件包进行配对t检验。结果:术后8周,所有3壁骨内缺损均有数量不等的新生牙槽骨、牙骨质和结缔组织。其中2个实验组的新生牙槽骨(3．7mm±0．3mm,2．3mm±0．2mm)与对照组(1．2mm±0．1mm)相比有显著性差异(P<0．01);实验组1与实验组2相比也有显著性差异(P<0．05)。而新生牙骨质和新生结缔组织附着在各组之间均无显著性差异(P>0．05)。结论:应用rh-bFGF／Bio-Oss胶原复合物更有利于牙槽骨再生,并且不会出现根吸收和骨固连等牙周再生治疗常见的不良结局。植入rh-bFGF／Bio-Oss胶原复合物对牙骨质再生和牙周韧带形成无显著作用。

PURPOSE： To evaluate the effectiveness of combined use of rh-bFGF and Bio-Oss collagen compounds on regeneration of the canine periodontal tissues. METHODS： Four adult hybrid canine were selected and bone defect of 5mm×5mm×5mm in size was created in the mesio and distal alveolar crest where the premolars were lost in both sides of the maxilla and mandible. Three methods were used in 3 groups,in the experimental group 1, rh-bFGF and Bio-Oss collagen compounds were implanted,Bio-Gide were overlaid;in the experimental group 2,Bio-Oss collagen compounds were implanted, Bio-Gide were overlaid;in the control group only Bio-Gide were overlaid. The therapeutic results were evaluated by clinical and imaging examination as well as histological index. SAS 6.2 software package was used for paired t test. RESULTS： 8 weeks post operation, there were various amounts of newly formed alveolar bone,cement and connective tissues in each 3-wall intrabony defect. There was significant difference in alveolar osteogenesis between the control group （1.2mm±0.1mm） and the 2 experimental groups （3.7mm±0.3mm, 2.3mm±0.2mm）.The difference was also significant between the two experimental groups （P〈0.01）. There was no significant difference in newly formed cement and connective tissues among the 3 groups （P〉0.05）. CONCLUSIONS： Application of rh-bFGF/ Bio-Oss collagen compounds benefits to the regeneration of the alveolar bone and avoid the side effects of root resorption ,bone malunion which often occur after conventional periodontal regeneration therapy.Addition of rh-bFGF/Bio-Oss collagen compounds was useless to cement genesis and periodontal ligament formation. Supported by Research Fund of Education Bureau of Liaoning Province（Grant No.202013124） and Natural Science Foundation of Liaoning Province （Grant No.20042062）.