江汉平原—规模化猪场暴发伪狂犬病的调查研究(Ⅲ)——猪伪狂犬病毒HS-9304株的克隆纯化及检定

Investigation of Aujeszry's Disease Occurred on a Pig Farm in Jianghan Plain (Ⅲ)——Clones and identification of aujeszry's virus strain HS-9304

应用细胞培养病毒蚀斑技术,将分离的伪狂犬病毒(PrV)HS-9304株在鸡胚成纤维细胞(CEF)单层培养上覆以营养琼脂培养基,连续挑斑纯化3次,成功地获得了纯化病毒克隆株.对病毒克隆株在传代细胞上复壮增殖后进行毒价测定.接种试验动物人工造病并进行血清中和试验以及外源性污染的检定.该病毒克隆株对BHK-21细胞感染的滴度为10^(8.5)TClD_50,能使小鼠和家兔出现特异性的症状和死亡,病毒对PrV标准阳性血清的中和效价达到1:195.病毒纯净无外源性污染.

With cell cutture virus plogue technotogy, the PrV strain HS-9304 isolated was Plaque-purified three limes in chick embryo fibroblast(CEF) covered by nulritional agar ne-dia, we obtained the purified virus cloned strain successfully. The slrain was strerglhened in passaging cell and its titer was delermined. inoculated little animal to reproductive illuess, did neutralizational test with serum and its outer contamination was examinated and determi-nated The titer of strain against cell infection was 108.5TCID50, Cauldmake mouce and rabbit enurge syntom and dead. The slrain has a neutralization titer of 1 : 195 against standard Positiue Serum , Without outer contamination.