摘要
TaLEA1基因是一个从小麦中克隆得到的编码胚胎晚期丰富蛋白的基因,用农杆菌EHA105(含卡那霉素抗性基因表达载体PBI121-TaLEA1)介导的叶盘转化法将其转入丹参(Salviamiltiorrhiza Bunge)组织培养苗中,在50mg/L卡那霉素选择压下连续筛选,并扩繁和生根培养,获得了7个丹参转基因株系。经PCR扩增,初步验证得到6个阳性株系,再继代4次后,Southern杂交结果表明已获得了一个能稳定遗传的转基因株系。该株系在含1%NaCl和8%PEG6000培养基中的生长状态优于野生型,表明转入TaLEA1基因可改善丹参组培苗的耐盐和耐旱性。
TaLEA1, a gene encoding a late-embryogenesis-abundant protein, was cloned from wheat and was transformed into Salvia Miltiorrhiza Bunge by Agrobacterium-mediated leaf-disk transformation method. Seven transgenic lines were obtained after kanamycin (50 mg/ L) screening. Six positive lines were obtained by PCR amplification, and after four additional generations, one stable line was obtained by Southern hybridization. Transgenic plants had better growth states than control plants on mediums containing 1% NaC1 and 8% PEG6000, which demonstrated that TaLEA1 played an important role in increasing the salt and drought tolerance of S. miltiorrhiza.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2007年第2期109-114,共6页
Journal Of Plant Physiology and Molecular Biology
基金
陕西师范大学校级重点基金(2004-2007)资助~~
关键词
丹参
农杆菌
TaLEA1基因
盐胁迫
干旱胁迫
Salvia miltiorrhiza Bunge
Agrobacteriumtumefaciens
TaLEA1 gene
salt stress
drought stress