水稻白叶枯病菌中超氧阴离子的非酶来源分析

Analysis of Nonenzymic Source of Superoxide Anion in Pathogenic Xanthomonas oryzae pv. oryzae

以电子自旋共振法及羟胺氧化法检测超氧阴离子,分析水稻白叶枯病原细菌OS-14细胞中超氧阴离子的来源。结果显示,OS-14细胞中的超氧阴离子释放活性主要位于胞外,因此胞外组分很可能为主要的释放位点。实验从多个角度排除了胞外组分中蛋白质酶类分子对超氧阴离子释放贡献的可能性,有机酸分子有可能是水稻白叶枯病菌OS-14细胞胞外组分中超氧阴离子释放的非酶分子。

With electron spin resonance and chemical assay, the production of superoxide anion in the Xanthomonas oryzae pv. oryzae strain OS-14 suspension is extracellularly localized （Figs. 1, 2）. It was found that superoxide anion produced in the filtrate. It was confirmed that peptide or enzyme did not contribute to the production of superoxide anion in the filtrate of OS-14 （Table 1, Figs.3, 4）. Organic acids were shown to be the primary source of superoxide anion produced in the filtrate of OS-14 （Fig.5）. These results indicated that non-enzymic molecules might be another source of superoxide anion produced by Xanthomonas oryzae pv. oryzae, in addition to enzymes, which suggested the multiform generation of superoxide anion.