CpG ODN107增强人脑胶质瘤细胞放射敏感性的研究

Study of CpG ODN107 on the radiosensitivity of human glioma cell line CHG-5

目的探讨CpG ODN107增加人脑胶质瘤细胞(CHG-5)放射敏感性的作用及可能机制。方法用不同浓度CpG ODN107处理CHG-5细胞,然后经放射线照射,MTT法检测细胞的增殖情况,体外培养集落形成率与细胞划痕法观察细胞的集落形成和迁移能力,ELISA法检测细胞TNF-α的分泌水平。结果CpG ODN107抑制CHG-5细胞的增殖,并呈量效关系。CpG ODN107(10μg/mL)联合放射线照射,抑制CHG-5细胞的增殖作用最强(0.52±0.76)。CpG ODN107(10μg/mL)同样能抑制CHG-5细胞增殖和迁移,并且增加了TNF-α的分泌,在联合照射后作用更加显著(193±0.58)pg/mL。结论CpG ODN107对CHG-5有明显的放射增敏作用,其机制可能与CpG ODN107增强肿瘤细胞TNF-α的分泌有关。

OBJECTIVE： To investigate the influence of CpG ODN107 on the radiosensitivity of human glioma cell line （CHG-5） and its mechanism. METHODS： CHG-5 was exposed to CpG ODN107 at different concentrations or/and irradiation, the abilities of proliferation were measured by MTT test, the abilities of colony formation and migration were observed by the colony formation test and scratch method, the TNF-α secretion by CHG-5 was detected by ELISA. RESULTS： CpG ODN107 dose-dependently inhibited the proliferation of CHG-5.and the inhibitory action was enhanced（0.52±0.76）when CpG ODN107（10μg/mL）was combined with β-ray irradiation.The colony formation and migration of CHG-5 were also inhibited by CpG ODN107（10μg/mL）or combined with irradiation ,with the increase of TNF-α seceretion by cells （193±0.58）pg/mL.CONCLUSION：Cpg ODN107 can increase the radiosensitivity of human glioma cell line （CHG-5）,which may be associated with enhancing TNF-α secretion by cells.