红河州鸡衣原体病的研究——病原分离鉴定和人工感染试验

Stidy on chlamydiosis in chicks in Honghe district:isolation and identification of the causative agents and artificial infection test

从采自发病或死亡小鸡的肝、脾、肺和腹水等病料中分离到４株衣原体。将分离物接种７日龄鸡胚卵黄囊，能致鸡胚规律性死亡；该分离株对磺胺嘧啶（ＳＤ）具有抵抗力，碘染色不着色，鉴定为鹦鹉热衣原体（Ｃｈｌａｍｙｄｉａｐｓｉｔａｃｉ）；毒力测定对鸡胚致死毒价为１０－１０ＥＬＤ５０／０．４ｍｌ。用该分离株对健康小雏做人工感染试验，可发生与自然病例相同的症状和病理变化。

Four strains chlamydia spp.were isolated from liver,spleen,lung and ascites specimens collected from the affected or dead chicks.When 7 days old chicken embryoes were inoculated with the isolates through a route of yolk sac,the embryoes were regularly killed;these isolates have resistance to sulfadiazine(SD) and arent pigmented post iodium staining,on basis of which the organisms are identified as Chlamydia psittaci.The lethal titer for chicken embryoes is 10 -10 ELD 50 /0.4 ml.The isolates were used to do an artificial infection test of normal poultry and the clinical signs and the pathological lesions in the test corresponded with the natural cases.At the same time the morphological features of the isolates were observed by an electron microscopy.