小麦转TPS基因植株的获得及其初步功能鉴定

Transgenic Wheat Plants with Trehalose-6-phosphate Synthase (TPS) Gene and Identification of Their Function

为了探索利用基因工程改良小麦抗旱性和耐盐性的途径,通过基因枪法将具有抗旱和耐盐碱功能的海藻糖合酶(TPS)基因导入普通小麦品种CB9945,获得了转TPS基因的小麦植株,对T0代植株进行了PCR检测,对T2代植株进行了叶片涂抹除草剂检测并进一步进行了验证,鉴定出15个转基因株系。采用模拟抗旱、耐盐环境,对15个转基因株系进行了初步功能鉴定,发现转TPS基因小麦植株的抗旱、耐盐能力得到了一定程度的提高。

It is very important to improve wheat tolerances to drought and salt by genetic engineering for its safe production. A trehalose-6-phosphate synthase （TPS） gene from Saccharomyces cerevisiae related to drought stress and salt tolerance of plant was transferred into CB9945, a common wheat variety, by biolistic bombardment. The To putative transgenic plants were tested by PCR, and one plant with TPS was identified. The progenies of T1 and T2 were detected by PCR and liberty detection on leaf, and 15 transgenic lines were obtained finally. Under simulated stress conditions of drought and salt, the transgenic lines were identified for their tolerance to stress, and the result indicated the stress tolerance of the transgenic plants was improved to some extent. The transgenic wheat has been sent to Shanxi province for drought test in field.