番荔枝内酯单体Desacetyluvaricin对肝癌细胞株生长和凋亡的影响

Effect of a Monomer Desacetyluvaricin of Annonaceous Acetogenin on Growth and Apoptosis of Hepatoma Carcinoma Cell Strain

【目的】探讨Desacetyluvaricin在体外对肝癌HpG2.2.15细胞株生长和凋亡的作用及机制。【方法】处于生长期的人肝癌HepG2.2.15细胞分为3组:对照组,Desacetyluvaricin组和顺铂组。用MTT法检测各组肿瘤细胞增殖情况,流式细胞仪检测各组细胞周期、细胞调亡以及凋亡基因Fas的表达情况,比较Desacetyluvaricin与顺铂的疗效。【结果】Desacetyluvaricin对肝癌细胞增殖的抑制率高达62.2%。Desacetyluvaricin抑制肝癌细胞增殖的机制可能为阻滞肝癌HepG2.2.15细胞从G1期进入S期,从而抑制了肿瘤细胞的增殖,同时通过促进Fas表达,诱导HepG2.2.15细胞凋亡。其诱导Fas表达的效果还优于顺铂。【结论】Desacetyluvaricin可能通过延缓细胞G1期及诱导细胞凋亡等机制,对肝癌细胞的增殖有一定的抑制作用。

[Objective] To investigate the effect of desacetyluvaricin on the growth and apoptosis of hepatoma carcinoma cell strain and its mechanism in vitro. [Method] Human hepatoma ceils HepG2.2.15 in proliferative phase were divided into three groups： Control group, Desacetyluvaricin group, and Cisplatin group. Proliferation of tumor ceils in each group was tested by MTT method. Cell cycle, cell apoptosis, and expression of Fas gene of tumor ceils in each group was detected by flow cytometer. Inhibitive effect on tumor ceils was compared between desacetyluvaricin and cisplatin [ Results ] The inhibitive rate on proliferation of hepatoma cells by desacetyluvaricin reached up to 62.2%. Parts of the intrinsic mechanism might relate to the blockage of HepG2.2.15 ceils to go to S period from G1 period thus to inhibit tumour cell proliferation, and the induction of HepG2.2.15 apoptosis by promoting expression of Fas gene. Besides, desacetyluvaricin showed more notable effect on promoting Fas gene expression compared with cisplatin. [Conclusion] Desacetyluvaricin can inhibit proliferation of hepatoma cells by prolonging G1 period of cell cycle, and inducing cell apoptosis.