利心丸对小鼠缺氧耐受的影响及机理

The effects and mechanism of Lixin Wan on the ability of anti-anoxia in mice

目的研究利心丸对小鼠缺氧耐受的影响并初步探讨其机理。方法小鼠随机分生理盐水(每天20g/kg)组、心得安(每天0.16g/kg)组、利心丸高剂量(每天5g/kg)和低剂量(每天1g/kg)组,连续给药5天并于末次给药1h后测定小鼠缺氧耐受能力以及血清乙酰胆碱酯酶(T-ChE)和总抗氧化能力(T-AOC)。结果利心丸低剂量组耗氧率和心得安组均较生理盐水组显著降低(P<0.05或0.01);心得安组T-ChE活性较生理盐水组显著增高(P<0.01);心得安组和利心丸高剂量组T-AOC均较生理盐水组显著增高(P均<0.01)。结论利心丸低剂量能提高小鼠抗常压的缺氧耐受能力,可能与提高机体总抗氧化能力有关。

Objective To investigate the effects and mechanisms of Lixin Wan （LXW） on the ability of antianoxia in mice. Methods The mice were randomly divided into four groups： normal saline （20g/kg daily） control group, propanolol （0.16g/kg daily） group, high dose LXW （5g/kg daily） group and low dose LXW （lg/kg daily） group. The mice were given medicine for 5 days and then the oxygen consumption rate （OCR） were measured after intragastric administration for one hour at the last day and the serum activity of T- ChE and T - AOC were also detected. Results In comparison with the saline control group, the OCR in low dose LXW group and propanolol group decreased significantly （ P 〈0.01 or 0.05）. The T-ChE activity in propanolol group elevated more significantly than normal saline group （ P 〈 0.01）. Compared to normal saline group, the T-AOC in propanolol group and high dose LXW group elevated more significantly （ P 〈0.01） Conclusion Low dose LXW can improve the ability of anti - anoxia in mice at normal pressure, it maybe associated with the up - regulating ability of total antioxygen capacity.