肥胖及超重儿童血浆抗氧化维生素的测定

DETERMINATION OF VITAMIN A、VITAMIN E IN PLASMA OF OBESITY AND OVERWEIGHT CHILDREN BY HIGH PERFORMANCE LIQUID CHROMATOGRAM

[目的]建立血浆中维生素A、维生素E含量测定方法。[方法]用高效液相色谱法进行分析。色谱柱为C18柱(5μm,4.6mm×250mm),流动相为甲醇︰水(98︰2),检测波长维生素A325nm、维生素E292nm。[结果]维生素A、维生素E分离良好,维生素A的浓度在0～10μg/ml,维生素E的浓度在0～15μg/ml的范围内有良好的线性(维生素A的R2=0.9997,维生素E的R2=0.9990);回收率在95%～103%之间(RSD﹤5.0%);日内、日间稳定性好(RSD﹤5.0%)。[结论]方法稳定,简便易行,快速准确,可作为血浆中维生素A、维生素E含量测定方法。

[Objective]To establish a HPLC method for determination of Vitamin A、Vitamin E in plasma.[Methods]HPLC method was used to analyze.The chromatographic colum was C18（5 μm,4.6 mm×250 mm）,the mobile phase was methanol-water（98︰2）.Detection wave length were 325nm for Vitamin A and 292nm for Vitamin E.[Results]Vitamin A、Vitamin E were separated well.The assay linearity was determined over the range of 0-10 μg/ml for Vitamin A and 0-15 μg/ml for Vitamin E（The R^2 of Vitamin A equalled to 0.9997,and the R2 of Vitamin E equalled to 0.9990） , The recovery rate varied from 95 % to 103% （RSD 〈 5.0%）,The Stability was also good （RSD 〈 5.0 %） [Conclusions] The method is simpiel rapid and accurate WhiCh can be applied to determinate Contents of Vitamin A, Vitamin E in plasma.