肝细胞癌组织中PTEN蛋白的表达以及与肝癌侵袭转移的关系

PTEN-protein Expression in Hepatocellular Carcinoma and its Association with Invasion and Metastasis

目的研究人原发性肝癌(HCC)中的磷酸酶基因(PTEN基因)蛋白的表达及其临床意义。方法用免疫组织化学方法分别检测人HCC组织、癌旁组织中PTEN蛋白的表达情况。结果PTEN蛋白的表达在人HCC组织显著低于癌旁组织(P=0.038),其阳性率也显著低于癌旁肝组织(P=0.007)。PTEN蛋白的表达与HCC患者的年龄、血清AFP水平、肿瘤个数、肿瘤直径、临床分期、术后复发、有无肝外转移、病理分级等多数临床病理学指标无关,而与有无门脉癌栓有密切的关系。无门脉癌栓的患者的肝癌组织中的PTEN蛋白较有门脉癌栓的表达高(P=0.010)。结论HCC的发生中可能存在PTEN基因的突变或缺失。PTEN基因的表达缺失可能与HCC的恶性程度,疾病的进程及HCC的侵袭转移有密切关系。PTEN基因低表达,HCC患者病程较晚、肝癌的恶性程度较高,较容易发生侵袭转移,预后较差。

Objective To study PTEN-protein expression in primary human hepatocellular carcinoma （HCC） and its clinical significance. Methods PTEN-protein expression in HCC and paraneoplastic tissues was analyzed by Immunohistochemistry. Results PTEN-protein expression levels and positive rates were significantly lower in human HCC than in paraneoplastic tissues. PTEN protein expression was not related to most of the clinicopathological feathers,including age, serum AFP level, number of tumors, size of tumor, clinical stage, local recurrence, distant metastases, and pathological grade. PTEN protein expression was related to portal tumor thrombus. Patients without portal tumor thrombus had higher PTEN protein expression than those with portal tumor thrombus （P = 0.010）. Conclusion PTEN gene mutation or loss of its expression may occur in the tumorogenesis of HCC. Loss of PTEN expression was closely related to the degree of malignancy of HCC, disease progression, and invasion and metastasis of HCC. Lower expression of PTEN tends to have more advanced disease, more maglinant tumor. Lower expression of PTEN is a negative prognostic factor.