HPLC法测定抗病毒口服液中盐酸麻黄碱的含量

Determination of Ephedrine Hydrochloride in Antiviral Oral Liquid by HPLC

目的：建立以HPLC法测定抗病毒口服液中盐酸麻黄碱含量的方法.方法：色谱柱为Diamonsil C18（250mm×4.6mm,5μm）,流动相为乙腈-0.1%磷酸（6∶94）,检测波长为207nm,流速为1.0mL·min^-1,柱温为室温.结果：盐酸麻黄碱进样量在5.8～29.0 μg范围内与峰面积积分值呈良好线性关系（r=0.999 6）;平均加样回收率为98.13%,RSD=1.40%（n=9）.结论：本法专属性强、灵敏度高、重现性好,可用于抗病毒口服液的质量控制.

OBJECTIVE： To establish a HPLC method for the determination of the content of Ephedrine hydrochloride in Antiviral oral liquid. METHODS： The determination was performed on Diamonsil C28（250mm × 4.6mm, 5μm） . The mobile phase consisted of acetonitrile-0.1% phosphate （6 ： 94） with flow rate of 1.0mL·min^-1. The detection wavelength was 207nm and the column temperature was set at room temperature. RESULTS： The linear range of Ephedrine hydrochloride was 5.8- 29.0μg （r = 0.999 6）, with average recovery at 98.13%, RSD = 1.4% （n - 9） . CONCLUSION： This method was highly specific, sensitive and reproducible, and applicable for the quality control of Antiviral oral liquid.