摘要
目的通过UVB照射导致的角朊细胞中丙二醛(MDA)、羟自由基(OH.)和超氧阴离子(O2-)水平的改变和清除这些物质酶类含量和活性的改变,探讨B段紫外线对角朊细胞的损伤作用及机制。方法实验组的角朊细胞用UVB进行照射,对照组细胞用普通日光灯照射。分别测定并比较实验组和对照组细胞产生MDA、OH.和O2-的水平以及两组之间谷胱甘肽过氧化物酶、超氧化物歧化酶的改变,探讨UVB导致角朊细胞的损伤作用及机制。结果实验组角朊细胞产生的MDA、OH.和O2-的水平显著高于对照组,P<0.05;实验组谷胱甘肽过氧化物酶、超氧化物歧化酶水平明显低于对照组,P<0.05。结论UVB照射能够导致角朊细胞的氧化损伤,使角朊细胞的的自由基产生增加,使谷胱甘肽过氧化物酶、超氧化物歧化酶水平明显降低。
Objective To explore the damages and mechanisms of UVB on keratinocytes through the measurement of MDA, OH·, O2^- ,glutathione oxidase and superexide dismutase level produced by kerafinocytes which were radiated by ultra-violet B.Methotis Keratinocytes in treated group were radiated by UVB and those in control were exposed to visible hght. MDA, OH·, O2^- level from two group keratinocytes were compared, so were the GSH and SOD contents in two groups. Results MDA, OH·, O2^- level in UVB treated group was much higher than those in visible exposed group;GSH and SOD were significantly lower in treated group than those in control. Conclusion UVB can cause oxidative damages to kerafinocytes and GSH,SOD contents decrease in treated group.
出处
《中国实验诊断学》
2007年第5期581-583,共3页
Chinese Journal of Laboratory Diagnosis
基金
中华医学会欧莱雅项目资助(S-2007040803)
关键词
角朊细胞
紫外线
氧化损伤
keratinocytes
oxidative damages
ultraviolet radiation