大鼠肾移植急性排斥反应病理演变研究

Pathological Change of Acute allograft rejection during renal transplantation

目的研究肾移植急性排斥反应早期移植物病理学改变,探讨肾移植急性排斥的发病机制。方法以LEW(RT11)大鼠为受体,LEW(RT11)和F344(RT11v1)大鼠为供体,建立大鼠原位肾移植急性排斥反应动物模型,移植后3、7、14天获取大鼠移植肾,利用HE染色观察同种同体和同种异体移植术后急性移植排斥反应早期病理演变情况。结果肾移植急性排斥早期,同种异体移植物中就出现了排斥反应病理的早期改变,其中以14天时更为明显,并且以细胞性排斥反应改变为主。结论肾移植急性排斥反应早期就出现了病理学改变,并且呈渐进性的发展趋势,对于今后发现亚临床的排斥反应及临界改变,为临床采取措施、及时调整治疗方案提供依据。

Objective To evaluate the changes of pathology in acute allograft rejection of renal transplantation and settle foundation for bringing out the pathogenesis. Methods Heterotopic kidney transplantation was performed using F344 or Lewis donors and Lewis recipients. No was used.graft kidneys were harvested on days 3,7,and 14.HE stained slides were made for observation and diagnosis. Results During the early period of post-transplantation, the changes of pathology became more and more serious, and major histopathologic change was cellular rejection. Conclusion Pathology changes appeard in the early period of post- transplantation and that is dynamic process. It will helps discover early subclinical rejection and borderline changes in patients with rehal transplantation, and beneficial for adjustment of immunosuppressive therapy.