抑瘤宁对体外培养MDA-MB-231、NCI-H520、A549、Hela细胞的影响

The Research on the Effect of Yiliuning on MDA-MB-231,NCI-H520,A549 and Hela Cells

目的研究抑瘤宁对体外培养MDA-MB-231、NCI-H520、A549、Hela细胞的生长抑制作用。方法采用MTT法检测抑瘤宁对体外培养MDA-MB-231、A549、NCI-H520、Hela细胞的生长抑制作用;采用流式细胞仪检测MDA-MB-231细胞周期分布及凋亡率。结果抑瘤宁可明显的抑制MDA-MB-231、NCI-H520、Hela、A549细胞的生长。流式细胞仪检测MDA-MB-231细胞周期分布及凋亡率结果显示,各实验组S期细胞百分率明显升高,G0-G1期细胞百分比明显下降,细胞被阻滞于S期,细胞凋亡率升高。结论抑瘤宁对MDA-MB-231细胞的增殖具有明显抑制作用,其机制可能与细胞周期阻滞和凋亡有关,对NCI-H520、A549细胞及人宫颈癌Hela细胞有明显的生长抑制作用。

Objective To study the proliferation inhibitory effect of Yiliuning on MDA-MB-231 ,NCI-H520,A549 and Hela cells. Methods By means of the MTT method to test the prohferation inhibitory effect of Yiliuning on MDA-MB-231,NCI-H520,A549, Hela ceils and by means of the flow cytometry to test the distribution of cell cycles and apoptosis rate on MDA-MB-231. Results It obviously inhibited the MDA-MB-231,NCI-H520,Hela,A549 cells＇ growth and the resuhs of flow cytometry analysis showed that the S-phase cells＇ percentage of the experimental group was obviously increased and the ceils＇ percentage from G0/G1 phase was obviously decreased ; and ceils were retarded in S-period, the percentage of cell apoptosis was increased. Conclusions The Yiliuning can inhibit MDA-MB-231 cells＇ proliferation, and its mechanism was relevant to the retardant of cells＇ cycle and apoptosis. It obviously inhibits NCI-H520,A549 and Hela cells＇ growth.