三氧化二砷和肼苯哒嗪体外诱导雌激素受体α表达强弱的比较

The Comparison of the Expression Effect of ER-α Induced by Arsenic Trioxide and Hydrazine

目的本文拟比较三氧化二砷和肼苯哒嗪两种去甲基化药物在最佳有效浓度时,诱导MDA-MB-231细胞株雌激素受体重新表达作用的差异。方法以雌激素受体高甲基化失活的人乳腺细胞系MDA-MB-231细胞系为研究对象,经2.0μmol/LAs2O3和10μmol/L肼苯哒嗪处理后,采用免疫组织化学技术检测ER-α受体的表达。结果经2.0μmol/L As2O3与10μmol/L肼苯哒嗪处理的MDA-MB-231细胞ER-α受体的表达差异无统计学意义(P>0.05)。结论两种去甲基化药物在各自最佳去甲基化作用浓度时,对于高甲基化失活的雌激素受体ER-α基因去甲基化作用差异无统计学意义。

Objective This present study was designed to compare the effect of re-expression of the silenced estrogen receptor （ER-α） gene in human breast cell line MDA-MB-231 by arsenic trioxide （As2O3） and hydrazine. Methods The MDA-MB-231 cell line in which the estrogen receptor gene was silenced due to hypermethylation was treated with 2.0 μmol/L As2 03 and 10 μmol/L hydrazine,the expression levels of ER-α genes were determined with immunohistochemistry technique. Results The detectable expression effect of ER-α protein had not significant difference by arsenic trioxide and hydrazine （P 〉 0.05）. Conclusions At their optimal concentration,the effect of re-expression of the silenced estrogen receptor gene was not different.