人胃癌VEGF-C、VEGF-D的表达与淋巴结转移的关系

The Expressions of VEGF-C and VEGF-D in Human Gastric Carcinoma and Their Relationship to Lymph Node Metastasis

目的通过从蛋白水平检测VEGF-C、VEGF-D在正常胃黏膜及胃癌中的表达情况,探讨胃癌发生淋巴结转移的机制。方法应用免疫组化SABC法检测VEGF-C、VEGF-D在正常胃黏膜及胃癌组织中的表达情况。结果VEGF-C在正常胃黏膜中阴性表达,在胃癌组织中有选择性的表达,阳性率为44.4%,胃癌组与正常胃黏膜组比较差异有统计学意义(P<0.01)。VEGF-D在正常胃黏膜中无表达,在胃癌组织中阳性率为41.3%,胃癌组与正常胃黏膜组比较差异有统计学意义(P<0.01)。在有淋巴结转移组和无淋巴结转移组,VEGF-C的阳性表达率分别为64.5%和25.0%,两组间比较差异有统计学意义(P<0.05)。VEGF-D在这两组中的阳性表达率分别为61.3%和21.9%,两组间比较差异有统计学意义(P<0.05)。且VEGF-C、VEGF-D的阳性表达与胃癌的组织学分级、浸润深度密切相关。结论VEGF-C、VEGF-D的过表达可能参与了胃癌的淋巴结转移。

Objective To investigate VEGF-C and VEGF-D expressions in gastric carcinoma and their relationship to lymph node metastasis. Methods SABC immunostaining technique was used to examine the expressions of VEGF-C and VEGF-D in normal gastric mucosa and gastric carcinoma. Results In the normal gastric mucosa, staining for VEGF-C protein was negative, and its positive rate was 44.4% in the gastric carcinoma group. There was significant difference in the expressions of VEGF-C between the normal mucosa group and the carcinoma group（P 〈 0.01 ）. There was no expressions of VEGF-D in the normal gastric mucosa, its positive rate was 41.3% in the gastric carcinoma group. There was significant difference in the expressions of VEGF-D between the two groups（ P 〈 0.01 ）. In the lymph node metastasis negative group and the lymph node metastasis positive group, the positive rates of VEGF-C were 64.5% and 25.0% respectively, and there was significant difference between the two groups（ P 〈 0.05） , the positive rates of VEGF-D in the two groups were 61.3% and 21.9% respectively, and there was significant difference between the two groups（ P 〈 0.05 ）. And the expressions of VEGF-C and VEGF-D were associated with the histological grade and the depth of gastric carcinoma invasion. Conclusions Over expressions of the two proteins may participate the lymph node metastasis of gastric carcinoma.