两歧双歧杆菌促生长培养基的初步筛选

Preliminary Screening of Enrichment Medium for Growth Promotion of B.bifidum

本试验首先比较了两歧双歧杆菌在TPY,PTYG和改良MRS培养基的生长情况,发现PTYG作为两歧双歧杆菌的生长培养基相对较好;然后在PTYG培养基中加入乳清蛋白水解液和乳糖,结果表明乳清蛋白水解液与乳糖对两歧双歧杆菌的生长有一定的影响,其适当添加量为10%乳清蛋白水解液(mL/mL)和1.5%乳糖(g/g)。

A comparison of the growth of on TPY, PTYG and modified MRS medium was made and PTYG medinm was shown to be the best one. Results also indicated that both the hydrolyzed whey protein and lactose could affect the growth of B. bifidum in PTYG medium and the most suitable additive amounts ofhydrolyzed whey protein and lactose were 10% （mL/mL） and 1.5% （g/g）, respectively.