蒲公英多糖的提取及含量测定

Extraction and Determination of the Polysaccharides from Dandelion

研究了蒲公英多糖的提取分离条件和对其含量进行测定。提取条件为料水比1:30,80℃保温3h,提取2次;分离条件为80%乙醇去除单糖和寡糖等杂质,Sevag法脱蛋白,石油醚回流脱脂,乙醇沉淀分离多糖;测定方法为蒽酮-浓硫酸法,测得的多糖含量为干重的52.06%,回收率为97%,RSD=2.06%,此法操作简便,重现性和稳定性都好,RSD=0.67%,适合蒲公英多糖的测定。

The extraction conditions of polysaccharides from dandelion roots were optimized here. The optimal extraction temperature, time, the ratio of solid to liquid and extraction times were 80℃, 3 h, 1：30 and 2 times, respectively. 80% ethanol was used to removal the impurities （monosaccharides and oligosaccharides） and to precipitate polysaccharides, while Sevag method and ligarine were used for deproteinization and degreasing, respectively, The content of polysaccharides was determined by spectrophotometric method with anthrone-sulfuric acid as coloring reagent and shown to be 52,06% with the recovery rate and RSD being of 97% and 2.06%, respectively. The method is suitable for the determination of dandelion polysaccharides due to its simpleness, convenience, accurateness and repeatability.