发酵法改进蒺藜皂甙提取工艺的研究

Improvement of the Extraction of Tribulus Terestris via Pre-fermentation

本文探讨了皂甙三氯乙酸封管水解和丁酮内标与紫外扫描结合法用于微生物发酵法改进蒺藜皂甙的提取。实验结果表明,经微生物发酵后再醇提工艺中的皂甙产率为单纯醇提工艺中皂甙产率的2.28倍。皂甙水解的最佳条件为:使用15%三氯乙酸水溶液,以丁酮为内标,封管120℃反应1h,产物用正己烷萃取,在207nm处读取吸光值。实验证明发酵法可明显提高蒺藜皂甙的提取率,而三氯乙酸封管水解法作为一种简便、可靠的分析方法,在皂甙研究领域具有良好的应用前景。

The hydrolysis of saponins in sealed tube with trichloride acetic acid using butanone as internal standard and monitoring with UV scanning were firstly applied in the extraction of Tribulus Terestris Saponins via pre-fermentation. The results showed that the extraction rate of Steroidal saponins via microorganism fermentation was 2.28 times higher than that without pre-fermentation. The optimal concentration of Trichloride acetic acid, intem＇,d standard, temperature for sealed tube and reaction time for the hydrolysis of Tribulus Tcrestris sapenins were 15%, butanone, 120~C and lh, respectively. The most suitable exWacting solvent was found to be n-hexane. Results showed that the extract rate of steroidal saponins was greatly improved after microorganism＇s fermentation. And the saponins hydrolysis in sealed tube with tdchloride acetic acid was shown to be a simple and reliable monitoring method with promising application prospect.