薄层扫描法测定微生物发酵转化液中熊果苷含量

Analysis of Arbutin in Microbe Fermentation Solution by Thin Layer Chromatography

目的:建立薄层扫描法测定微生物发酵转化液中熊果苷含量的方法。方法:微生物发酵转化液经过滤浓缩制备,以乙酸乙酯-乙酸-水(v乙酸乙酯:v乙酸:v水=3.1:1:1)为展开剂,检测波长570nm。结果:熊果苷在2～6μg范围内线性良好,相关系数r=0.9976。结论:本法快捷、简便、准确,可用于微生物发酵转化液中熊果苷含量的测定。

Objective： To establish a method for determination of the content of arbutin in microbe conversion solution by Thin-Layer chromatography. Methods： The microbe conversion solution was filtrated and condensed. The developing solvent was a mixture of ethyl acetate, acetic acid and water （3.1：1：1, v/v/v）. The detection wavelength was set at 570 nm. Results： The calibration curve was Lincar in the range of 2μLg-6μg with r value of 0.9976. Conclusions： The method was quick, simple and accurate for the determination of content of arbutin.