低能量氦-氖激光对周围神经再生的影响

INFLUENCE OF LOW ENERGY HE NE LASER ON REGENERATION OF PERIPHERAL NERVE/

用４４只体重２．５ｋｇ的家兔作实验，分４，８，１２及１６周４个观察期，照射组各用兔６只，对照组各用兔５只。麻醉后，均切断左侧腓总神经，用９／０尼龙单丝对端吻合神经外膜。照射组在术后１ｄ开始用８３１８－Ｂ型低能量氦－氖激光仪经皮肤照射Ｌ５，６脊髓节段，每天照射１５ｍｉｎ，共照射１４ｄ。对照组不照射，均按期观察。结果表明：术后４周，可在照射组看到细小而稀少的再生轴突，对照组直到术后８周才能看到（Ｐ＜０．０１）。腓总神经潜速率，照射组均优于对照组（Ｐ＜０．０１）。术后１６周照射组大多数的再生轴突被覆有明显髓鞘，对照组则较少。胫前肌肌纤维横纹，照射组在１６周时很清楚，对照组不甚清楚。展趾功能到术后１６周时，照射组与健侧相同，对照组才恢复到照射组１２周的水平。证明，低能量氦－氖激光促进了脊髓运动神经细胞功能，加快了轴突再生的速度。

The purpose of this experiment was to elucidate the influence of the low energy He Ne laser on the function of regeneration of peripheral nerve. Forty four rabbits about 2.5kg body weight were used in the experiment. The animals were divided into 4, 8, 12, 16 weeks groups according to the observation period. Six animals were used in each irradiated group and in the control group 5 rabbits were used in each observation period. regeneration of the axon and myelinc sheath, the latent rate of the common peroneal nerve, the conditions of the anterior tibital muscle and the toe expansion test were all observed systematically in both groups. The experimental results was: A few thin regenerated axon was seen at 4 weeks in the irradiated group, while in the control group it might be seen at 8 weeks, the P value was <0.01. A low amplitude latent rate of the common peroneal nerve is determined at the peroneal side of the anterior tibial muscle in a few animal at 4 weeks of the irradiated group, and it is not observed in the control group, from 12 to 16 weeks. THe latent rate of the common peroneal nerve was the irradiated group than in the controlled, the P value was <0.01. The regeneration of the myeline sheath was evident in the irradiated group, and also the slstion of the musdle fibers anterior tibial muscle was clearly visible than the controlled. 16 weeks postoperatively, the toe expansion test was normal in the irradiated group, while in the control group it was the same as seen at 12 weeks after operation in the irradiated group. Now it was certain that the low energy He Ne laser could promole the function of the spinal motor nerve cells and accelerate the axonal regeneration.