鼻咽癌放射敏感性与MDR1基因多态性的关系

Relationship between radiosensitivity of nasopharyngeal carcinoma and MDR1 gene polymorphism

目的探讨多药耐药基因(MDR1)多态性(21外显子G2677T和26外显子C3435T)与鼻咽癌放射敏感性差异的相关性。方法对59例行根治性放疗的鼻咽癌病人,采用DNA限制性片断长度多态性法测定其MDR1G2677T、C3435T和两者的单体型,并用基因测序法验证。结果C3435TCC基因型放射敏感性显著高于CT和TT基因型(P=0.026);G2677TGG基因型行根治性放疗的疗效优于GT和TT基因型,但差异无显著性;携带有2677G-3435C单体型的病人放射敏感性显著强于其他单体型携带者(P=0.017)。结论MDR1G2677T和C3435T多态性可能是鼻咽癌根治性放疗疗效的影响因素之一。

Objective To investigate the relationship between human multidrug resistancel gene （MDRI） polymorphisms and the radiosensitivity of nasopharyngeal carcinoma （NPC）. Methods Blood samples were collected from 59 NPC patients, who were devided into radiosensitive or radioresistant groups according to their responses to radiation therapy. The genotypes for MDR1 polymorphisms （G2677T in exon 21 and C3435T in exon 26） and their haplotypes were determined by PCR and restriction fragment length polymorphism analysis. The results were further confirmed by sequencing. Results The 3435CC genotype was associated with a significantly better response to radiotherapy than combined 3435 CT and TT genotype （P=0. 026）. The 2677GG genotype was also associated with a better response in comparison with combined 2677 GT and TT genotype, but this relation was not statistically significant. Patients with 2677G-3435C haplotype had a significant better response to radiotherapy than those with the other haplotypes （P--0.017）. Conclusion The MDRI G2677T and C3435T polymorphisms may help predict the response to radiotherapy in NPC patients.