摘要
目的以人成骨细胞(MG-63细胞)复合纳米羟基磷灰石/胶原/聚乳酸[nano-hydroxyapatite/collagen/poly(L-lactic)acid,nHAC/PLA]支架材料进行体外培养,观察其早期附着生长情况。方法将MG-63细胞在nHAC/PLA支架材料上培养,通过倒置显微镜观察、HE染色、ALP染色、细胞增殖指数测定等方法对细胞在nHAC/PLA支架材料上的早期附着情况进行研究。结果细胞在nHAC/PLA上生长良好;ALP染色阳性度高,细胞增殖指数比空白对照组有显著提高。结论nHAC/PLA支架有利于MG-63细胞的早期黏附、生长,可以用作骨组织工程的支架材料。
Objective To study the early adhesion of human osteoblast on nano-hydroxyapatite/collagen/Poly （L-lactic） acid （nHAC/PLA） scaffold material. Methods Human osteoblast were seeded on nHAC/PLA scaffold material and cultured in vitro. The culture process was observed by inverted phase contrast microscope,and HE staining. The alkaline phosphatase （ALP） staining and proliferation index of cells were measured to study the effect of nHAC/PLA scaffold material on human osteoblasts. Results The spreading and proliferation of osteoblast cells throughout the inner- pores of the scaffold material were observed. There was significant difference of the proliferation index of co-cultured ceils, compare to the contral group. Conclusion The nHAC/PLA scaffold material has no effect on the spreading and adherence of MG-63 ceils and it can be used as bone tissue engineering scaffold material.
出处
《生物医学工程与临床》
CAS
2007年第3期161-163,F0003,共4页
Biomedical Engineering and Clinical Medicine
基金
辽宁省教育厅资助项目(05L481)
