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混合菌群的筛选及其与白腐真菌串联降解石油的效果初探 被引量:6

The Screening of Mixed Microbial Consortium and Its Associating Degradation Effect on Crude Oil with A White Rot Fungi
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摘要 从江苏油田提供的7个采集点的油污土样中筛选得到石油降解率较高的6株细菌——X-5、X-12、X-13、X-21、X-22、X-23和1株真菌——Z-26,通过两两混合实验发现X-23与X-5、Z-26具有较好的协同作用,将三者按1∶1∶1组成得到石油降解率较高的混合菌群,其石油降解率为75.9%。为进一步降低残留的石油含量,引入了1株白腐真菌进行后期处理,使得水相中石油含量降低,颜色明显变浅。利用气相色谱分析经混合菌和白腐真菌降解前后石油成分的变化,结果发现,石油中正构烷烃得到了有效的降解,除C17和C18外,其它正构烷烃峰峰值均明显降低,甚至消失。 A mixed microbial consortium was screened from six petroleum-biodegradation bacterial strains (X-5, X-12, X-13, X-21, X-22, X-23 ) and a petroleum-biodegradation fungus ( Z-26 ), which had been obtained from Jiangsu Oil Field's oil-polluted soils by the mixed experiment of two strains randomly. The results showed that X-5, X-23 and Z-26 could degrade 75.9 % of crude oil effectively after mixed. The mycoderma produced by a white rot fungi was added into the oil culture, which had been treated by the above three strains. It revealed that the residual content of petroleum could reduce. Due to the good adsorptivity of the mycoderma and its special degradation mechanism, the petroleum content of aqueous phase was decreased obviously. And the gas chromatography test indicated that n-alkanes could be removed absolutely whose molecular weights were higher than 18-alkane.
出处 《化学与生物工程》 CAS 2007年第5期34-37,共4页 Chemistry & Bioengineering
关键词 混合菌群 白腐真菌 石油 串联降解 mixed microbial consortium white rot fungi petroleum associating degradation
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