HPLC法测定昆明山海棠蜜中雷公藤甲素的含量

Determine Triptolide in Triptergium hypoglaucum Honey by HPLC

昆明山海棠蜜经萃取洗脱后，采用反相C^18柱，以乙腈-水（30：70）为流动相，以218nm为检测波长，检测昆明山海棠蜜中雷公藤甲素的含量。结果：雷公藤甲素在1～20μg／g范围内线性良好，r为0．9999，平均回收率为103．06％（n=5），RSD为1．71％。

After elute Triptergium hypoglaucum honey, use reversed-phase C^18 column determine triptolide, with acetonitrile-water（30： 70） as mobile phase, 218nm as determine wave. Result shows that Triptolide has a good linearity within 1 μg/g - 20 μg/g, and the r - value is 0. 999 9, the mean recovery is 103.06% （n =5）, RSD is 1.71%.