高效毛细管电泳分析羊栖菜多糖对肿瘤机体红细胞合淌度的影响

Effect of Sargassum fusiforme Polysaccharides on the Complex Mobility of Erythrocytes in Tumor-Bearing Organisms Using High Performance Capillary Electrophoresis

为了分析羊栖菜多糖(SFPS)对肿瘤机体红细胞合淌度的影响,建立了肿瘤动物模型,分高、中、低剂量腹腔给予羊栖菜多糖7d,采集并制备红细胞悬液,应用高效毛细管电泳法检测红细胞的合淌度。实验条件:毛细管为75μm×50cm,电泳缓冲液为含2g/L羟丙基甲基纤维素的磷酸盐溶液(0.1mol/L,pH7.4),压力进样为3.448kPa×10s,分离电压为20kV,柱温为25℃,电渗淌度为2.16×10-4cm2.V-1.s-1。实验结果表明:羊栖菜多糖能降低肿瘤机体红细胞的电泳迁移时间(阴性对照组为15.76min,SFPS低剂量组为13.96min,SFPS中剂量组为12.90min,SFPS高剂量组为13.51min,正常对照组为11.51min),增加红细胞的合淌度(阴性对照组为1.06×10-4cm2.V-1.s-1,SFPS低剂量组为1.19×10-4cm2.V-1.s-1,SFPS中剂量组为1.29×10-4cm2.V-1.s-1,SFPS高剂量组为1.23×10-4cm2.V-1.s-1,正常对照组为1.45×10-4cm2.V-1.s-1),SFPS3个剂量组红细胞的合淌度与阴性对照组比较均有非常显著的差异(P<0.01)。羊栖菜多糖能够改变肿瘤机体红细胞的合淌度,并使之趋向于正常机体水平,这可能与其改变红细胞表面的电荷密度有关。高效毛细管电泳法可以作为检测红细胞生理状态和功能的一种辅助工具。

This study analyzed the effect of Sargassum fusiforme polysaccharides （SFPS） on the complex mobility of erythrocytes in tumor-bearing organisms. A mouse tumor model was developed in which mice were intraperitoneally injected with high, medium, and low dosages of SFPS for 7 d. The erythrocytes were collected and prepared into suspensions, and the complex mobilities of cells were measured using high performance capillary electrophoresis （HPCE）. Experimental conditions included the following： capillaries, 75 um × 50 cm; buffer for electrophoresis, phosphate solution containing hydroxypropylmethyl cellulose （0. 1 mol/L, pH 7. 4 ） ; injection pressure, 3. 448 kPa; injection time, 10 s; separation voltage, 20 kV; column temperature, 25 ℃. The electroosmotic mobility was calculated to be 2.16 × 10^-4 cm^2· , V^-1 · s^ -1 It was found that SFPS can decrease the migration time of erythrocytes in tumor-bearing organisms, which was 17. 56 rain for the control group, 13.96 min for the low dosage group, 12.90 min for the medium dosage group, 13.51 min for the high dosage group, and 11.51 min for the normal group, and increase its complex mobility, which was 1.06 × 10^-4 cm^2 · V^-1 · s^-1 for the control group, 1.19 × 10^-4 cm^2 · V^-1 · s^-1 for the low dosage group, 1.29 ×10^-4 cm^2 · V^-1 ·s^-1 for the medium dosage group, 1.23 × 10^-4 cm^2 · V^-1 · s^-1 for the high dosage group, and 1.45 × 10^-4 cm^2 · V^-1 · s^-1 for the normal group. Compared with the negative control group, all three dosage groups had significant differences （P 〈0.01 ） in the complex mobility of erythrocytes. It was found that SFPS can change the complex mobility of erythrocytes in tumor-bearing organisms with the tendency to the condition in normal organisms, which is possibly because SFPS can change the charges density on erythrocytes surface. It is believed that HPCE can be used as an auxiliary tool for determining the physiological state and functions of erythrocytes.