具有油水分配系数测算功能的中药指纹图谱

A Functional Fingerprint of Traditional Chinese Medicine for Measuring the Oil-Water Partition Coefficients

建立了一种能同时测算中药中主要成分的油水分配系数Pow的功能化中药指纹图谱。采用微乳液电动色谱,以中药女贞子为例,对运行缓冲液浓度、pH值、十二烷基硫酸钠浓度等参数进行了优化。利用SPSS程序对磺胺等从女贞子中提取的6种标准化合物组分的迁移时间tm与其logPow文献值进行非线性拟合,得到女贞子提取组分的标准方程线性关系良好(r=0.988 0)。在相同的实验条件下测得化合物的logPow与文献报道值较好的符合,证明该方法可靠。获得了能代表组分疏水化学特征的中药指纹图谱,测得女贞子的主要有效成分齐墩果酸的logPow为3.63。该指纹图谱较为可靠地提供了女贞子中各种成分的logPow,直观地反映出各组分油水分配性质的分布,对中药有效成分提取溶剂的选择、剂型的设计及制剂工艺的改进具有指导意义。

Following the principle and method on the measurement of oil-water partition coefficients by microemulsion electrokinetic chromatography （MEEKC）, a traditional Chinese medicine fingerprint （TCM-FP） was established. The components which were extracted from the TCM were separated from each other by MEEKC under optimized operation conditions. The conditions of running solution were of 70 mmol/L SDS, 87 mmol/L n-heptane, 814 mmol/L nbutanol and 5 mmol/L sodium tetraborate with pH value of 8.80. The uncoated fused silica capillary used was 54 cm × 75 um with an effective length of 45 cm. The UV detection was carried out at 210 nm and an electrophoretic voltage of 15 kV was applied. SPSS program was used for the nonlinear regression of migration time and log Pow values of the standard compounds from literature, and the reliability of the established method has been investigated. As a result, the standard equation of regression with good linearity （ r = 0. 988 0 ） was obtained, The log Pow values of compounds measured under the same operation conditions were well consistent with those from literature. This method was applied to L. lucidum in which oleanolic acid is a main effective component. Besides the establishment of its TCM-FP, the log Pow of oleanolic acid, 3.63, was also obtained. The functional fingerprint of traditional Chinese medicine can give the log Pow of the extracts from TCM, reflect oil-water partition character of the constituents, and is of great significance to the selection of solvents, the design of dosage form, and the improvement of production technology.