不结球白菜BrCBF基因cDNA全序列克隆及结构特征分析

Cloning and characterization of full length cDNA of BrCBF gene from Brassica campestris ssp.chinensis

运用RT-PCR和RACE技术从不结球白菜中克隆到1个抗寒相关基因,命名为BrCBF,基因登陆号为DQ402470。其cDNA全长1 003 bp,含有648 bp的完整开放阅读框,编码216个氨基酸,该基因编码的蛋白序列与拟南芥、荠菜、甘蓝型油菜编码的氨基酸序列有极高的同源性,且具有CBF典型的保守结构域AP2。

With RT-PCR and RACE strategies, the full-length cDNA gene named BrCBF, a gene related to cold resistance （ accession number DQ402470） was cloned from Brassica campestris ssp. chinensis. The length of its cDNA was 1 003 bp, which encompassed an open reading frame （ORF） with 648 bp encoding 216 amino acid residues. The protein encoded by this gene was highly homologous with that of Arabidopsis thaliana, Capsella bursa-pastoris and Brassica napus. Furthermore, the gene contained a conserved domain of AP2, which is typical of CBF.