生长抑素DNA疫苗对湖羊羔羊生长和相关激素的作用——佐剂效应

Effect of the somatostatin DNA vaccine on growth and relative hormones in Hu lambs:adjuvant effect

选择60只断奶湖羊母羔羊,分为6组,1-5组为免疫组,分别用pES/2SS＋pE-CpG、pES/2SS＋细菌DNA、pES/2SS-GMCSF、pES/2SS脂质体和pES/2SS免疫,第6组为对照组。结果显示,首次免疫4周和加强免疫2周后,细菌DNA佐剂组的抗生长抑素抗体水平显著高于pES/2SS单独免疫组（P〈0.05）,其抗体阳性率也最高,达50%。比较全期14周免疫羊的体质量增加情况,最优为pE-CpG佐剂组,其次为细菌DNA佐剂组,分别比对照组高33.0%和31.6%（P〈0.01）,且显著高于pES/2SS单独免疫组（P〈0.05）。加强免疫2周后,免疫组GH和IGF-Ⅰ水平显著高于对照组（P〈0.05）,抗体阳性羊的GH和IGF-Ⅰ水平极显著高于抗体阴性羊（P〈0.01）。结果表明：几种佐剂配合pES/2SS免疫,产生了免疫佐剂效应,能增强生长抑素DNA疫苗的免疫效果。

Sixty female Hu lambs were divided into six groups to immunized with different adjuvant such as the pE-CpG plasm plasmid, E. coil DH5α, the crude liposome were combined with pES/2SS, pES/2SS-GMCSF, pES/2SS and the control group. The results showed that the level of antibody of immunized group was remarkably increased compared with the control （ P〈0.05 ）. The antibody against SS in the E. coil DH5α group was remarkably higher than that in the pES/2SS alone at week 4 after primary immunization and week 2 after booster. The sheep immunized with the E. coil DH5α group got the highest rate of positive antibody （50%）. During the whole experimental period （14 weeks） , body weight gains in the pE-CpG and the E. coil DHSαgroups were 33.0% and 31.6%, respectively, which were higher than that of the control （P 〈0.01 ） and pES/2SS alone （P 〈 0.05）. Two weeks after the booster, the level of serum GH and IGF - Ⅰ in the immunized group were remarkably higher than those in the control （P 〈0.05）, and those with positive antibody higher than negative antibody （ P〈0.01 ）. These results suggest that different adjuvant are combined with pES/2SS, can produce immunity adjuvant affect, have an enhancement effect on the SS DNA vaccine.