饲料中铅对鸡胚肝脏抗氧化能力的影响

Effect of different Pb contents on the hepatic anti-oxidative capacity of chicken embryo

将80只伊莎褐蛋种鸡随机分为4组,在饲料中分别添加15、30和60 mg.kg-1铅,以全价饲料(铅含量为3.55mg.kg-1)为对照组,研究种蛋中铅对鸡胚肝脏抗氧化能力的影响。收集饲喂第25天的种蛋进行孵化,孵化9、14和19 d测定鸡胚肝脏的丙二醛含量和相关抗氧化酶活性。结果表明,9 d时仅有谷胱甘肽-S-转移酶(GST)随着铅含量的增加而显著降低(P<0.05),14 d时铅的添加量基本不影响各指标的变化,当鸡胚生长到19 d时铅的添加显著降低了丙二醛含量,过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)和GST活力显著下降,但19 d时除CAT外其他指标的改变都与铅添加量无关。总之,铅对鸡胚胎肝脏的损伤中,脂质抗氧化作用并不是其主要原因,主要是由于相关抗氧化酶活力改变所致,同时饲料中铅在60 mg.kg-1以下时,其含量对肝脏的抗氧化作用影响不显著。

In this paper, a total of eighty 30-week-laying hen （ ISA Brown） was randomly divided into 4 groups. Different contents of lead （Pb） （ 15, 30 and 60 mg·kg^-1 ） was added into feedstuffs with regular feedstuff as control group （ 3. 55 mg·kg^-1）. Hepatic anti-oxidative capacity of chicken embryos was studied. Eggs of the 25 th day were collected and hatched. The hepatic MDA and relative anti-oxidative enzymes of 9 d, 14 d and 19 d chicken embryos were detected. For the 9 d chicken embryos, only GST activity was significantly decreased with the increasing of Pb contents （ P 〈 0. 05 ） ; for 14 d embryos, various indexes were not influenced by Pb and its contents. Only for the 19 d embryos, the adding of Pb significantly reduced the production of MDA and the activities of CAT, SOD, GR and GST, whereas all indexes except for CAT were affected by the content of Pb. In a word, liver injury was resulted from the change of enzyme activities, but not from lipid peroxidation. At the same time, the content of Pb lower than 60 mg ·kg^-1,in feedstuff did not significantly influence the anti-oxidative function of liver.