金草片对子宫肌瘤模型大鼠子宫组织一氧化氮合酶表达的影响

Effects of Jincao Tablet on Expression of Nitric Oxide Synthase in the Model of Uterine Leiomyoma in Rat

目的:探讨金草片对子宫肌瘤模型大鼠子宫组织一氧化氮合酶(NOS)表达的影响,及该模型与临床发病机理的相关性。方法:以雌激素和雌、孕激素肌肉注射造成大鼠子宫肌瘤模型,测定子宫组织的总型NOS和诱导型NOS(iNOS)的水平;用放射配体结合分析法测定子宫组织雌激素受体(ER)水平;用放射免疫法测定血清雌二醇(E2)和孕酮(P)水平。结果:模型组大鼠子宫组织总型NOS和诱导型iNOS表达均明显增强,E2、ER和P水平升高;P水平与NOS表达相关,与iNOS表达不直接相关。金草片组可明显降低子宫组织NOSi、NOS和ER表达,降低血清E2和P水平,与模型组比较差异显著。结论:金草片可抑制子宫肌瘤模型大鼠子宫组织NOS表达,其作用可能是该品种治疗子宫肌瘤的作用机理之一。

Objective：To investigate effects of Jincao tablet on expression of nitric oxide synthase in the model of uterine leiomyoma in rat and the relationship between the model and pathogenesis. Method： Rats were injected with diethyl stilbestrol and progesterone respecti,~ely. The expression of NOS and iNOS in uterine leiomyoma tissue were measured by kit;the levels of ER in uterine leiomyoma tissue was assayed by radioligand combination assay;the levels of E2 and P in serum was determined by radioimmunity. Result： Compared with normal group, the levels of and NOS, iNOS, ER, E2 and P of model group were significantly decreased. The levels of P were related to NOS and were not related to iNOS. Jincao tablet group was obviously different from model group. Conclusion： Jincao tablet could improved the levels of immune function of the model of uterine leiomyoma in rat, and it might be played a role in the pathogenesis of leiomyoma.