血管紧张素转化酶基因的插入/缺失多态性与心肌梗塞关系的研究

The relation hip between insertion/deletion polymorphism of angiotensin converting enzyme gene and myocardial infarction in a Chinese population

本研究运用ＰＣＲ技术对我国人１１６例心肌梗塞（ＭＩ）和１０３例健康作对照的血管紧张素转化酶（ＡＣＥ）基因插入／缺失多态性进行了检测，并与血清ＡＣＥ水平、ＭＩ发病、冠状动脉病变支数等指标进行比较。结果显示ＭＩ组缺失等位基因Ｄ频率０．４６和ＤＤ基因型频率０．２７显著高于对照组的０．３３和０．１２（分别为Ｐ＜０．０１，Ｐ＜０．０５）。同时发现缺失多态性与血清ＡＣＥ水平、ＭＩ组冠状动脉病变支数呈相关性。表明ＡＣＥ基因缺失多态性可能是我国人群ＭＩ发病的重要危险因素之一。

Objective: Angiotensin converting enzyme(ACE) is a key component of the renin angiotensin system(RAS). It was reported that insertion/deletion (I/D)polymorphism of the ACE gene was associated with serum ACE level and myocardial infarction(MI) morbidity in Caucasian population. This study aims to investigate the relations hip between the I/D polymorphism and MI as well as serum ACE level in a Chinese population. Methods: I/D polymorphism in intron 16 of the ACE gene was determined by polymerase chain reaction(PCR) in a study of 103 control subjects and 116 patients with MI, among which 45 patients were angiographically documented. Serum ACE levels were concomitantly measured. Results: The frequencies of D allele(0.46) and DD genotype(0.27)were higher among the MI group than among the control subjects(0.33 and 0.12 respectively, P <0.01 and P <0.05). With the increment of the D allele frequency in the genotypes, serum ACE levels increased progressively in the MI group without ACE inhibitors medication and control subjects. Furthermore, multivessel disease was more strongly associated with D allele( P <0.05). Conclusion: Deletion polymorphism of the ACE gene is associated with serum ACE level and MI morbidity, indicating that it might be an important risk factor for MI in Chinese population.