慢性胃炎胃粘膜组织幽门螺杆菌感染与π类谷胱甘肽S-转移酶的相关性研究

Study on the Relationship Between Helicobacter Pylori-infectionand and Glutathione S-Transferase-π in Gastric Mucosa with Chronic Gastritis

目的:探讨谷胱甘肽S-转移酶(GST-π)在慢性胃炎持续幽门螺杆菌(HPylori)感染胃粘膜组织中的变化及其与HPylori感染的关系。方法:将内镜及胃粘膜活检病理证实的138例慢性胃炎(其中慢性浅表性胃炎96例,慢性萎缩性胃炎42例),分别用13C呼气实验及病理切片改良Giemsa染色检测HPylori,按HPylori检测结果分为HPylori阳性组(87例)及HPylori阴性组(51例);用S-P免疫组化检测GST-π,观察与比较两组间胃粘膜组织中GST-π的表达,计算各组GST-π表达率,将所得结果输入spss统计学软件进行统计学处理。结果:本研究HPylori阳性87例,平均阳性率63.04%;GST-π阳性共58例,平均阳性率为42.01%。其中慢性浅表性胃炎组HPylori阳性61例,阳性率为63.54%;慢性萎缩性胃炎组HPylori阳性26例,阳性率为61.91%,两组间HPylori阳性率无显著差异(χ2=-0.0006,P>0.05)。慢性浅表性胃炎组GST-π阳性37例,阳性率为38.54%;萎缩性胃炎组GST-π阳性21例,阳性率50%,两组间阳性率无显著差异(χ2=1.14,P>0.25)。两组合并后HPylori阳性亚组中GST-π阳性率为32.18%显著低于HPylori阴性亚组的GST-π阳性率58.82%(χ2=8.30,P<0.005)。结论:持续HPylori感染可降低胃粘膜GST-π的表达,削弱GST-π对胃粘膜损伤的保护作用。

Objective： To observe the expression of glutathione S-transferase-π（GST-π） in gastric mucosa with chronic gastritis and Hp infection,and analyze virulence effect of Helicobacter pylori （H Pylori）in the development of gastric mucosal damage. Methods： 138 patients with chronic gastritis confirmed by endoscope and pathology were taken in this study（including 96 patients with chronic superficial gastritis and 42 cases with chronic atrophic gastritis）. H Pylori infection was confirmed by 13 C breath test and histological examination on biopsy specimens with Giemsa dyeing,GST-π were detected by S-P immunochemiohistology. The patients were divided into two groups they were H Pylori positive group including 87 cases, H Pylori negative group including 51 cases. The data were analyzed to assess statistical differences with X^2 test. Results： There were 87 cases with H Pylori positive in this study,the positive rate of GST-π expression was 32.18% in the H Pylori positive group,which was significantly lower than that in H Pylori negative group（58. 82 %, X^2=8. 3, P〈0. 005）, the GST-π expression rate in H Pylori positive two subgroups was lower significantly than that of two H Pylori negative subgroups and in either of two chronic gastritis groups too the average GST-π expres-sion rate in chronic atrophic gastritis mucosa was higher than that in chronic superficial gastritis but without statistical significance（38.54% vs 50% ,X^2 = 1.14, P〉0.25） the H Pylori positive rates of the two groups chronic superficial gastritis group and chronic atrophic gastritis group,were 63.54% and 61.91% respectively and there was no significant difference（X^2 = -0. 0006 ,P〉0.05）. Conclusion： Expression of GST-π was significant lower in H Pylori positive than that in H Pylori negative the findings indicate that continous H Pylori infection may decrease expression of GST-π,and impair defensive function of GST-π.