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牛血浆纤维黏连蛋白抑制大鼠成骨细胞凋亡的实验研究

Experimental study on bovine plasma fibronectin suppressing apoptosis of rat osteoblasts in vitro
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摘要 目的研究外源性牛血浆纤维黏连蛋白(fibronectin,FN)对体外培养的大鼠成骨细胞凋亡的影响。方法采用体外培养技术原代培养大鼠成骨细胞,Western blotting法检测FN作用后成骨细胞凋亡相关基因Bcl-2及Bax的蛋白表达变化。结果体外培养的成骨细胞呈梭形、三角形或多角形,多角形细胞有多个矢状突起,核卵圆形常居一侧,Ⅰ型胶原免疫组化染色呈阳性。FN作用后细胞凋亡相关基因Bcl-2的蛋白表达增强;Bax基因的蛋白表达减弱。结论一定浓度的牛血浆FN能够抑制成骨细胞凋亡,其机制之一是增强大鼠成骨细胞胞浆内Bcl-2基因所表达的蛋白,同时减弱Bax基因的蛋白表达。 Objective To investigate the effects of bovine plasma fibronectin on the apoptosis of rat osteoblasts in vitro. Methods Cells were cultured. Western blotting was used to determine the effects of various concentrations of bovine plasma fibronectin on the expression of Bcl-2 and Bax protein of osteoblasts in vitro.Results Fibronectin can increase the protein expression of Bcl-2 and decrease the protein expression of Bax. Conclusions Certain concentrations of bovine plasma fibronectin may suppress apoptosis of rat osteoblasts in vitro, which attributes to the increase of the protein expression of Bcl-2 and decrease the protein expression of Bax.
作者 薛明 侯志明 张颖 艾红军
机构地区 中国医科大学口腔医学院口腔内科
出处 《口腔医学》 CAS 2007年第5期252-254,共3页 Stomatology
基金 辽宁省科技攻关计划项目(2005225003-2)
关键词 纤维黏连蛋白 成骨细胞 细胞培养 凋亡 鼠 fibronectin osteoblasts cell culture apoptosis rats
分类号 R329.28 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

  • 1Yin XM,Ottval ZN,Korameyer SJ.BH1 and BH2 apoptosis and heterodimerization with Bax[J].Nature,1994,369(6478):321-323.
  • 2Oltvai ZN,Milliman CL,Korameyer SJ.Bcl-2 heterodimerizes in vivo with a conserved homolog,Bax,that accelerates programmed cell death[J].Cell,1993,74(4):609-619.
  • 3Shim JW,Koh HC,Chang MY,et al.Enhanced in vitro midbrain dopamine neuron differentiation,dopaminergic function,neurite outgrowth,and 1-methyl-4-phenylpyridium resistance in mouse embryonic stem cells overexpressing Bcl-XL[J].J Neurosci,2004,24(4):843-852.
  • 4Karim Kaabeche,Hind Guenou,Daniel Bouvard,et al.Cbl-mediated ubiquitination of alpha5 integrin subunit mediates fibronectin-dependent osteoblast detachment and apoptosis induced by FGFR2 activation[J].J Cell Sci,2005,118(Pt 6):1223-1232.
  • 5Fuente MT,Casanova B,Gila MG,et al.Fibronectin interaction with α4β1 integrin prevents apoptosis in B cell chronic lymphocytic leukemia:correlation with Bcl-2 and Bax[J].Leukemia,1999,13(2):266-274.
  • 6Boudreau N,Sympson CJ,Werb Z,et al.Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix[J].Science,1995,267(5199):891-893.
  • 7Zhang ZH,Vuori K,Reed JC,et al.The α5β1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression[J].Proc Natl Acad Sci USA,1995,92(13):6161-6165.
  • 8Scott G,Cassidy L,Busacco A.Fibronectin suppresses apoptosis in normal human melanocytes through an integrin-dependent mechanism[J].J Invest Dermatol,1997,108(2):147-153.
  • 9吴勇,陈元仲,吕联煌.纤维连接蛋白对内毒素血症小鼠保护作用的实验研究[J].中国病理生理杂志,2003,19(6):799-802. 被引量：5
  • 10郑燕芳,吕海.纤维粘连蛋白与整合素结合调控肿瘤细胞的凋亡[J].中国癌症杂志,2003,13(5):441-444. 被引量：2

二级参考文献41

  • 1谭琛 罗正曜.休克与细胞凋亡[A].见:罗正曜主编.休克学 第1版[C].天津:天津科学技术出版社,2001.542—551.
  • 2Saba TM, Jaffe E. Plasma fibronectin (opsonic glycoprotein) :its synthesis by vascular endothelial cells and role in cardiopulmonary integrity after trauma as related to reticuloendothelial function[J]. Am J Med, 1980, 68(4): 577- 594.
  • 3Lotem J, Cragoe EJ Jr, Sachs L, et al. Rescue from programmed cell death in leukemic and normal myeloid cells[ J ]. Blood,1991, 75(4) : 953 - 960.
  • 4Chen YZ, Gu XF, Caen JP, et al. Interleukin- 3 is an autocrine growth factor of human megakaryoblasts, the DAMI and MEG- 01 cells[J]. Br J Haematol, 1994, 88(3):481 -487.
  • 5Leist M, Gammer F, Bohlinger I, et al. Tumor necrosis factorinduced hepatocyte apoptosis precedes liver failure in experimental murine shock models [J]. Am J Pathol, 1995, 146(5) : 1220- 1234.
  • 6Johnston CA, Finkelstein JN, Gelein R, et al. Pulmonary cytokine and chemokine mRNA levels after inhalation of lipopolysaeeharide in C57BL/6 miee[J]. Toxieol Sci, 1998,46(2) : 300 - 307.
  • 7Flohe S, Dominguez Fernandez E, Ackermann M, et al. Endotoxin tolerlance in rats: expression of TNFα, IL- 6, IL-10, VCAM- 1 and HSPT0 in lung and liver during endotoxin shock[J]. Cytokine, 1998, 490(10): 796-804.
  • 8Ortiz A, Alonso J, Gomez- Chiarr M, et al. Fibronecfin decreases glomerular lesions and synthesis of turnout necrosis fac-tor- alpha, platelet activating factor and in proliferative glomerulonephrifis[ J ]. Clin Exp Immunol, 1995, 101 (2) :334- 340.
  • 9Zhang Z, Vurri K, Reed JC, et al. The a5β1 integrin supports survival of cells on fibronectin and up - regulates BCL- 2 expression[J]. Pmc Natl Acad Sci USA, 1995, 92(11): 6161- 6165.
  • 10Wielockx B, Lannoy K, Shapiros D, et al. Inhibition of matrix metalloproteinases blocks lethal hepatitis and apoptosis induced by tumor necrosis factor and allows safe antitumor therapy [ J ].Nat Med, 2001, 7(11): 1202- 1208.

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口腔医学
2007年 第5期

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