重组人甲状旁腺激素1-34对骨质疏松性骨折愈合影响的实验研究

Effects of recombinant human parathyroid 1-34 on healing of osteoporotic fractures

目的研究重组人甲状旁腺激素1-34（rhPTH1-34）片段对大鼠骨质疏松性骨折愈合的影响。方法选择6个月龄雌性SD大鼠80只，随机分为rhPTH1-34组、雌激素组、对照组（骨质疏松组）及假手术组，每组20只。前三组切除双侧卵巢，假手术组暴露卵巢而不切除，术后3个月行右侧股骨中段骨折内固定术。术后分别皮下注射rhPTH1-34、苯甲酸雌二醇及等量生理盐水，进行骨密度、X线片组织病理学和生物力学检测，观察骨折愈合情况。结果rhPTH1-34组骨折局部骨密度明显高于对照组，差异有统计学意义（P〈0．05）；rhPTH1-34组比同时期的对照组骨痂生成量多，愈合时间提前。结论rhPTH1-34能促进骨形成，增加骨量，加快骨痂形成，促进骨质疏松性骨折愈合，同时能提高骨生物力学特性和抗骨折能力。

Objective To research the effects of recombinant human parathyroid 1-34 （rhPTH1-34） on healing of osteoporotic fractures in rats. Methods Eighty female SD rats of 6 months old were randomly divided into 4 equal groups： rhPTH1-34 group, estrogen group, osteoporosis group （control） and sham-operation group. Internal fixation of fractures of right mid femur was performed for the former 3 groups 3 months after bilateral ovariectomy. Subcutaneous injections of rhPTH1-34, oestradiol benzoate and physiologic saline were conducted respectively after fixation. BMD, X-ray, histopathological analysis of callus and biomechanicaltests were used to evaluate the bone healing. Results The BMD and biomechanical property in the rhPTH1-34 group were significantly superior to those in the other 3 groups 9 weeks after treatment （ P 〈 0.05） . Conclusion rhPTH1-34 can stimulate the growth of bone, increase the BMD, and promote the fracture healing in ovariectomized rats.