西维来司钠对实验性大鼠脑缺血再灌注损伤的保护作用

Protective effect of sivelestat sodium on ischemia reperfusion brain injury in rats

目的:研究西维来司钠对实验性大鼠脑缺血再灌注损伤的保护作用,并探讨其作用机制。方法:体外培养大鼠神经元,以缺氧加缺糖培养建立神经元“缺血”性损伤模型,观察西维来司钠对培养神经元“缺血”性损伤的保护作用。结果:西维来司钠(10-8,10-7及10-6mol/L)能明显增高模型细胞的活性;能显著降低模型细胞乳酸脱氢酶(LDH)漏出率;明显降低裂解液中MDA含量,提高SOD活性。结论:西维来司钠对大鼠脑神经元“缺血”损伤具有保护作用,其机制可能与其抗过氧化作用有关。

Objective：To explore the protective effect of sivelestat sodium on cerebral ischemia reperfusion in rats and its mechanism. Methods：The neurons of neonate rat were cultured in vitro.The protective effects of sivelestat sodium on ischemia injury were observed by treating cells in glucose-free and oxygen-free medium.Results：Sivelestat sodium （10^-8,10^-7 and 10^-6nol/L）increased the activity of neurons,reduced the leakage rate of LDH,increased the activity of SOD and reduced the content of MDA.Conclusion：sivelestat sodium protects the neurons against ischemia injury by suppressing the generation of lipid peroxide.