制川乌煎煮时间和给药剂量与抗炎镇痛功效的相关性研究

Study on the Correlationship between Decoction Time,Administration Dose and the Efficacy of Resisting Inflammation and Relieving Pain of Prepared Sichuan Aconite Root

目的研究制川乌煎煮时间、给药剂量与抗炎镇痛功效的相关性,确定药物发挥功效的最佳煎煮时间和给药剂量。方法根据川乌祛风除湿止痛的功效,选择热板痛证模型、扭体痛证模型、急性炎证模型、亚急性炎症模型等,运用均匀设计和回归分析方法,确定药物不同功效的最佳煎煮时间和最佳给药剂量。结果制川乌能明显抑制二甲苯所致小鼠耳廓肿胀;显著减少醋酸所致小鼠扭体次数,延长小鼠扭体潜伏期;明显延长热板刺激小鼠舔足潜伏期,提高热板小鼠痛阈值。制川乌发挥抗炎功效的最佳煎煮时间为6 h,最佳给药剂量为2.4 g/kg;镇痛功效的最佳煎煮时间为6 h,最佳给药剂量为2.4 g/kg。结论制川乌具有明显的抗炎作用和中枢镇痛作用;煎煮时间、给药剂量与抗炎镇痛功效显著相关。发挥抗炎镇痛功效的最佳煎煮时间和剂量为6 h,2.4 g/kg。

Objective To investigate the correlationship between decoction time （DT）, administration dose （AD） and the actions of resisting inflammation and relieving pain of Prepared Sichuan Aconite Root （ PSAR ）, confirm the best DT and AD of the PSAR acting. Methods According to the efficacy of the PSAR dispelling wind and dampness and relieving pain, several animal models, including pain, inflammation models, uniform design （UD） and regression analysis were applied to confirm the best DT and AD of its different actions. Results The PSAR obviously suppressed the mice＇s auricle swelling induced by xylene, reduced the number of mice＇s spraining body induced by acetic acid, increased their latencies of spraining body and stimulation of hot board, and improved their pain threshold value. The best DT and AD for the PSAR to resist inflammation and relieve pain were 6 hours and 2.4 g/kg respectively. Conclusion The PSAR possesses evident actions of resisting inflammation and relieving pain, which are markedly correlated with the DT and AD. The best DT and AD for PSAR to resist inflammation and relieve pain are 6 hours and 2.4 g/kg respectively.