摘要
目的建立筋骨痛消丸中丹参酮ⅡA的高效液相色谱测定方法。方法用二氯甲烷-甲醇(4∶1)提取样品,ODS-C18色谱柱,甲醇-水(80∶20)为流动相,流速0.8m l/min,检测波长270nm,外标法定量分析。结果分离度1.8,RSD=1.59%,平均回收率104.6%。结论该法分离度、精密度及稳定性好,可作为筋骨消痛丸的质量控制方法。
Objective To establish an HPLC method for the determination of thanshinone ⅡA in Jingutongxiao Pills. Methods Chloroform -methanol （4：1 ） was used as the solvent to prepare the samples. ODS -C18 was used as stationary phase. Methanol -water （80： 20） was used as the fluid phase. The flow rate was 0.8 ml/min. The detection wavelength was 270 nm. Quantitatively analytic method was external standard means. Results The resolution was 1.8, RSD was 1.59%. The average recovery was 104.6%. Conclusion The results showed the method has good resolution precision and accuracy. The method can be used for the quality control of Jingutongxiao Pills.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第5期1051-1051,共1页
Lishizhen Medicine and Materia Medica Research
基金
河南省科技发展计划项目(No.997015900)
