HPLC测定强力宁片中紫丁香苷和甜菜碱的含量

Determination of syringin and betaine in Qianglining Tablet by HPLC

目的：建立强力宁片（刺五加、枸杞子）中紫丁香苷和甜菜碱的含量测定方法。方法：紫丁香苷采用Hypersil C18柱,甲醇-0.1 mol/L醋酸溶液（24∶76）为流动相,检测波长为270 nm。甜菜碱采用Hypersil NH2柱,乙腈-水（25∶75）为流动相,蒸发光检测器。结果：紫丁香苷在0.121 6～0.608 0μg呈良好的线性范围（r=0.999 9）;平均回收率为99.09%,RSD为2.13%。甜菜碱在0.660 8～3.964 8μg呈良好的线性范围（r=0.999 1）;平均回收率为96.34%,RSD为1.31%。结论：该方法简便准确,重现性好,可用于强力宁片的质量控制。

AIM： To establish the HPLC method for determinating syringin and betaine in Qianglining Tablet （Radix et Rhizoma seu Caulis Acanthopanacis senticosi, Fructus Lycii ）. METHODS： A Hypersil C18 column was used for the determination of syringin. The mobile phase for syringin was methanol-0. 1 mol/L acetic acid （24： 76）, and the detection wavelength was at 270 nm. A Hypersil NH2 column was used for the determination of betaine. The mobile phase for betaine was acetonitrile-water （25： 75 ）, and ELS detector was used. RESULTS ： The linear range of syringin and betaine were 0. 121 6-0. 608 μg （ r = 0. 999 9） and 0. 660 8-3. 964 8 μg （ r =0.999 1 ）, respectively. The average recoveries of syringin and betaine were 99.09% （ RSD = 2. 13% ） and 96.34% （RSD = 1.31% ）, respectively. CONCLUSION： The method is simple, accurate, and can be used for the quality control of Qianglining Tablet.