液相色谱-串联质谱法测定大鼠血浆中莪术醇的浓度

Determination of Curcumol Concentration in Rat Plasma by Liquid Chromatography-tandem Mass Spectrometry

建立了一个快速、准确、灵敏的液相色谱-串联质谱测定鼠血浆中莪术醇的分析方法．血浆样品经乙腈沉淀蛋白后，以甲醇-乙腈-10mmol／L乙酸铵溶液（体积比：42．5：42．5：15）为流动相，采用反相色谱柱Nueleosil C18分离，通过电喷雾离子化源的四极杆串联质谱仪，以多反应监测方式检测．线性范围为2．5～500．0μg／L，日间和日内精密度〈4．79％，平均提取回收率为100．7％．

A rapid, simple and sensitive liquid chromatography/tandem mass spectrometry （LC/MS/MS） method was developed for the determination of eureumol in rat plasma. Samples of the plasma were deproteinated with aeetonitrile, the analytes were then analyzed by reversed-phase HPLC on a Hypersil ODS column with methanol-aeetonitrile-10 mmol/L ammonium acetate （volume ratio： 42.5 ： 42.5 ： 15 ） as mobile phase. Detection was carried out by multiple reaction monitoring on a API4000 LC/MS/MS system with an eleetrospray ionization （ESI） interface. The assay was linear in a range of 2.5 - 500.0μg/L, intra- and inter-day precisions over this range were 〈 4.79%, the average recovery was 100.7%.