外周血单个核细胞二维凝胶电泳图谱的建立

Establishment of 2-dimensional electrophoresis maps of peripheral blood mononuclear cells

目的:利用蛋白质组学方法建立肝细胞癌(hepatocellular carcinoma,HCC)及正常人外周血单个核细胞(peripheral blood mononuclear cells,PBMC)的双向凝胶电泳图谱。方法:利用固相pH梯度双向凝胶电泳分离HCC及正常人PBMC的总蛋白质,凝胶经蓝银显色后,采用PDQuest图像分析软件进行比较分析。结果:得到了分辨率较高、重复性较好的HCC及正常人PBMC双向凝胶电泳图谱,平均蛋白质点数分别为1206±48及1123±37;组内的平均匹配率分别为90.8%及92.6%。结论:建立了分辨率高且重复性较好的HCC及正常人PBMC双向凝胶电泳图谱,为寻找恶性肿瘤早期诊断、治疗及预后监测的有效指标提供了一条新的途径。

Objective To establish the 2-dimensional electrophoresis （2-DE） profiles of peripheral blood mononuclear cells （ PBMC ） in patients with hepatocellular carcinoma （ HCC ） and health adults. Methods The total proteins from PBMC in patients with HCC and healthy adult were seperated by immobilized pH gradient-based 2-DE. The differential expression proteins were analyzed by PDQuest analysis software. Results The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults were obtained. For HCC, the average spots of 2-DE maps were 1 206 ± 48, and the average matching rate was 90.8 %. For normal adluts, the average spots were 1 123 ± 37, and the average matching rate was 92.6%. Conclusion The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthly adults are established. These proteomic analysis methods are useful to screen the potential biomarkers in the early diagnosis, treatment and prognosis monitor in patients with malignant tumor.