3品种鹅Myostatin基因3′-调控区SNPs群体遗传学分析

SNPs Analysis of 3′-Regulatory Region of Myostatin Gene in Three Geese Breeds

利用已测得的鹅Myostatin基因3′-调控区核苷酸序列设计5对PCR-SSCP引物,对扬州鹅、皖西白鹅以及五龙鹅3品种家鹅该区域内单核苷酸多态性进行了分析,并探索了单核苷酸多态性与生产性能之间的关系。结果表明,检测到A→G(88位)和G→T(353位)2个单碱基突变位点。88位处,E等位基因为优势等位基因,具有EE、EF两种基因型,以扬州鹅杂合子频率最高(0.550),皖西白鹅30个个体仅有一例EF型;353位处,G为优势等位基因,具有GG、GH和HH 3种基因型,3品种均具有较高的GG基因型频率、较低的HH基因型频率。屠体性能分析发现,EF基因型家鹅具有更高的屠体性能;扬州鹅、皖西白鹅GG型个体的腿肌重和腿肌率显著高于HH型个体(P<0.05),五龙鹅腿肌重、腿肌率具有GG型(GH型(HH型的趋势,但差异不显著(P>0.05)。

5 pairs of primers were designed to identify SNPs in 3＇-regulatory region of Myostatin gene in 3 geese breeds, and the relativity of SNPs and slaughter characters was also analyzed. And the results showed that two SNPs were detected, which were A88G and G353T. Population genetic analysis indicated that there were EE and EF genotypes in 88 bp site, and E allele was a superior one. The frequency of EF genotype was especially high in Yangzhou geese （0. 550）, but very low in Wanxi White geese （0. 033）. In 353 bp site, three genotypes, GG, GH and HH were detected . The frequency of GG was the highest and that of HH was the lowest in 3 geese groups. The relativity analysis showed that individuals with EF had higher slaughter performances than those with EE. For leg muscle weight（LMW） and leg muscle percent（LMP） , individuals with GG were significantly higher than those with HH in Yangzhou geese and Wanxi White geese （P 〈0.05）.