反10,顺12共轭亚油酸对肉仔鸡肉品质和相关酶活的影响

Effect of Supplemental trans-10,cis-12 Conjugated Linoleic Acid on Growth Performance,Meat Quality and Relative Enzyme Activities in Broiler Chicks

选择1 d健康AA肉仔鸡288只,研究不同水平反10,顺12共轭亚油酸(t-10,-c12 CLA)对肉仔鸡生长性能、肉品质、相关激素和酶活性的影响。试验采用单因素随机设计,将试验鸡随机分成6组,每组6个重复,每个重复8只鸡,分别饲喂0、0.20%、0.40%、0.60%、0.80%和1.00%t-10,-c12 CLA的玉米-豆粕型饲粮,试验期42 d。结果表明,饲粮CLA对肉鸡全期日增重、饲料转化率有显著影响(P<0.05),极显著降低了腹脂率(P<0.01),对采食量、胸肌率、腿肌率、胸肌和全净膛率均无显著影响(P>0.05);饲粮CLA对肉鸡肉色b*值、剪切力和胸肌的品尝评价有显著影响(P<0.05),对pH、肉色L*、a*值和腿肌品尝评价无显著影响(P>0.05);饲粮CLA对肉鸡血清胰岛素l、eptin(P<0.05)和组织中苹果酸脱氢酶(P<0.01)有显著影响。饲粮t-10,c-12 CLA可提高肉仔鸡的饲料转化率和日增重,对肉品质剪切力和品尝有改善作用;CLA降低腹脂率,增加了肌内脂肪的含量,可能与leptin浓度和苹果酸脱氢酶活性有关。

An experiment was conducted using a total of 288 one day-old Arbor Acres male broilers to study the effect of supplemental t-10, c-12 CLA levels on growth performance, carcass traits, and abdominal fat of the broilers. Chicks were randomly allotted by body weight to one of six treatments in a completely randomized design. They were fed either a CLA-unsupplemented corn-soybean meal basal diet or fed basal diets supplemented with 0.20%, 0.40%, 0.60%, 0. 80% or 1.00 % t-10, c-12 CLA for duration of 42 days. The supplemental CLA had no effect （P 〉0.05） on the ADFI, percentage of breast and leg muscles,but affected significantly abdominal fat percentage （P〈 0.01）, ADG and F/G（P〈0.05）. The addition of 0.20%, 0.40%, 0.60%, 0.80% or 1.00 % CLA resulted in a significant decrease in abdominal fat percentage （P〈0.01）, and broilers fed on the 0.60% t-10, c-12 CLA had a higher ADG and F/G （P%0.05）. The addition of 0.20 %, 0.40%, 0.60% or 1.00% CLA decreased b ＊ value and shear force in the breast muscle （P〈0.05）, but not in the thigh muscle. The addition of CLA increased significantly taste estimate in breast muscle. The addition of 0.20%0, 0. 40%, 0. 60% or 1. 00% CLA increased MDH activity in abdominal fat, but decreased significantly MDH activity and leptin （P〈0. 05）. Results from this study indicated that the addition of t-10, c-12 CLA to broiler diets might increase growth performance and feed conversion, decrease the percentage of abdominal fat by decreasing MDH activity in abdominal fat and leptin in serum, increasing MDH activity in liver, breast and thigh muscle.